November/December 2016 | Page 39

Answer: A. Squamous papilloma (SP). A. Squamous papilloma (SP). The squamous papilloma is a very common benign epithelial lesion of the oral cavity, induced by the human papilloma virus (HPV). There are at least 150 types of the HPV virus.1 The most common types of HPV involved in the pathogenesis of SP are types 6 and 11, which have a low risk of transformation, low virulence, and low infectivity rate.2 The exact mechanism of transmission has, therefore, not been fully elucidated but may occur through direct contact with another lesion. SP represents about 3 percent of all oral biopsies submitted, and occurs in one of every 250 adults. It represents almost 7 percent of all oral soft tissue growths in children.2 The lesion is common on the soft palate and tongue, but can also develop on any mucosal surface in the oral cavity, including the labial mucosa. It can develop at any age and in any gender, although it is most often seen in patients between the ages of 30-50 years.2 Clinically, SP presents as an asymptomatic, exophytic, pebbly, rough, pedunculated mass or nodule with numerous papillary projections. The papillary projections can be blunted or pointed, which can give it a “wart-like” or “cauliflower-like” appearance. The lesion is usually solitary, and its color may range from normal mucosal color to slightly red or white in appearance, depending on the amount of keratinization. The lesion can exhibit rapid growth and ranges in size from 0.5 to upwards of 3.0 cm in diameter.2 Following the initial growth phase, the lesion remains stable in size. Multiple lesions associated with HPV can present in patients with immunosuppression, such as post-transplant, HIV-associated, or chemotherapeutic immunosuppression.3 The presence of multiple SPs that coalesce may also be seen in certain skin disorders and syndromes, such as acanthosis nigricans, Down syndrome, and focal dermal hypoplasia. Another condition caused by HPV types 6 and 11 is recurrent respiratory papillomatosis (RRP).2 RRP can show extensive, and potentially devastating, disease of the larynx and respiratory tract. RRP can present in children (juvenile onset), often via infection from the mother through the birth canal, amniotic fluid, or placenta, or in adulthood (adult onset). This condition can give rise to massive papillary growths in the respiratory tract and can lead to difficulty breathing. Treatment is typically based on symptoms, and the lesions often grow back after surgical excision. Histologically, SP demonstrates papillary projections of parakeratinized stratified squamous epithelium with fibrovascular connective tissue cores (Figure 2a and 2b). Often noted is an increase in mitotic activity and hyperplasia of the basal cell layer, which could be mistaken for mild epithelial dysplasia.3 (Figure 3). Koilocytes (koilo-, hollow or concave) are epithelial cells with pyknotic (darkly staining) nuclei surrounded by a clear halo.2 They are often noted in the spinous cell layer of the epithelium, and are seen in approximately 50 percent of SPs.3 Koilocytes represent HPV-infected epithelial cells. Truly atypical features, including dysplasia and invasion, are worrisome for malignant transformation, although this is rare.3 There is no convincing association of SP with development of oral squamous cell carcinoma. Treatment involves conservative surgical excision, and recurrence is unlikely. Given that SPs are the most common papillary lesions to affect intraoral mucosa, and based on the clinical manifestation of this lesion as a solitary, pedunculated, exophytic lesion with finger-like projections, a diagnosis of SP was favored in this case. However, a biopsy was never performed and the patient was lost to follow-up. Therefore, other possibilities cannot be fully excluded. These are described below. Figure 2A and 2B: (H&E, 20x and 40x): Low and medium power views show an epithelial proliferation with papillary projections. Figure 3: (H&E, 400x): A high power view shows epithelium and vascular connective tissue cores. Increased mitotic activity is noted in the basal cell layer, a finding frequently noted in SP. N O V / D E C 2 0 1 6 | P E N N S Y LVA N I A D E N TA L J O U R N A L 37