November/December 2016 | Page 40

DISCUSSION B. Verruca vulgaris (VV). VV is a benign hyperplasia of stratified squamous epithelium and, like SP, is an HPV-driven process. The most common HPV type involved in the pathogenesis of VV is type 2. VV is a very common skin lesion, especially in children (average age 13), although it can develop at any age. A slightly higher predilection for girls has been reported.4 The lesion is considered contagious. It can spread by auto-inoculation, as well as spread to others via direct contact. Oral lesions are rare in comparison to skin lesions, and in comparison to oral SPs. When it does present intraorally, VV s most commonly found on the labial mucosa, vermilion border, or anterior tongue.2 VV can present as a solitary lesion, or multiple lesions may develop. It is not uncommon for clusters of lesions to be present. VV most commonly presents as a painless, exophytic growth with finger-like papillary projections, or it may exhibit a pebbly, rough surface. Lesions can be painful if they are located in an area that is easily irritated.4 VV can be either sessile or pedunculated, and the color can vary from white or pink, to yellowish. Oral lesions are almost always well-keratinized and white in appearance. The lesion can exhibit rapid growth to its maximum size, which is usually less than 5mm in diameter. Once it has reached its maximum size, the lesion usually remains unchanged in size, unless irritated.2 When irritated, the lesion may grow. Histologically, VV contains papillary, finger-like projections with underlying connective tissue cores that may resemble SP. (Figure 4a and 4b) However, VV shows epithelium that is hyperkeratotic with alternating para- and orthokeratin and a prominent granul ar cell layer on H&E stain. The rete ridges of the epithelium are elongated and converge towards the center, producing a characteristic “cupping” effect.5 Koilocytes are often noted in the superficial epithelium. Eosinophilic intranuclear viral inclusions may also be noted in the granular cell layer.2 A chronic inflammatory infiltrate may or may not be seen in the underlying connective tissue. Treatment of cutaneous lesions involves topical medications, such as salicylic acid, lactic acid, or cryotherapy using liquid nitrogen. Salicylic acid is the most effective; however it is slow to work and requires frequent applications, for up to 12 weeks.6 Cryotherapy is successful 50-70 percent of the time.6 Oral lesions are most often treated with surgical excision that should include the base of the lesion. Alternative treatment methods have also been tried with varying degrees of success, including intralesional immunotherapy, intralesional bleomycin or 5-fluorouracil, and photodynamic therapy. Combination therapy is a more effective approach in many cases.4 Recurrence can occur in a small percentage of treated cases. VV has not been reported to undergo malignant transformation. If left alone, up to two-thirds of cutaneous lesions will actually spontaneously regress within two years. Spontaneous resolution is more commonly seen in children.2 In the present case, the lesion was located in the oral cavity, which placed VV lower on the list of differentials, since the lesion is relatively rare intraorally. Additionally, the patient had no reported history of skin VV. The lesion in our case was solitary and relatively large, also favoring an SP over a VV. However, VV should be considered in the differential diagnosis. C. Condyloma acuminatum (CA). CA is a common, benign, HPV-induced, sexually-transmitted lesion that occasionally presents in the oral cavity. Overall, CA affects about 1 percent of the sexually active population.2 CA presents more commonly in the anogenital region, but it can be seen in the mouth and the larynx. In some cases, it may be difficult to detect intraorally.7 Ninety percent of CAs are caused by HPV types 6 and 11.2 The virus has an incubation period of 1-3 months before a clinically apparent lesion develops, but it can remain dormant for longer periods.8 When seen in young children, they may be an indicator of abuse or may be due to vertical transmission through the birth canal in an infected mother. Nearly one half of the new infections occur in those 15-24 years old, but it can present in all age groups.8 It should be noted that the introduction of the HPV vaccine has dramatically reduced the incidence of CA in adolescents and young adults.7 The most common intraoral sites are the labial mucosa, lingual frenum, and soft palate. Lesions are frequently multiple and clustered. CA presents as a pink, sessile, non-tender, exophyic, well-demarcated mass with short blunted surface projections. Lesions can range in size from 1.0 to 3.0cm.2 Histologically, CA presents with papillary projections that are more broad and Figure 4A and 4B: (H&E, 200x and 100x): Hyperparakeratinized finger-like projections with fibrovascular cores seen in a SP. It does not show alternating orthokeratinization and parakeratinization, as seen in VV. 38 N O V / D E C 2 0 1 6 | P E N N S Y LVA N I A D E N TA L J O U R N A L