Effect of H 2 O 2 on [ Ca 2 + ] |
To determine whether changes in intracellular calcium are mediated by a specific calcium channel was added to 10 µ M verapamil cardiac cells before treatment with H 2 O 2. Fluorescence was measured. |
a d b c a 12 µ M H 2 O 2
b 24 µ M H 2 O 2 c 48 µ M H 2 O 2 d Control
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● The levels of [ Ca 2 + ] are dependent on the dose of H 2 O 2.
● The Verapamil if it inhibits the increase in [ Ca 2 + ].
● Comparing these results with those obtained with LEVL indicates that 12 µ M H 2 O 2 can mimic the effect of 3.6 J / cm 2 of visible light and both cause a slow and lasting [ Ca 2 + ].
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c b a a 18 µ M H 2 O 2
b 18 µ M H 2 O 2 + 10 µ M Verapamil c 10 µ M Verapamil
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Effect of light or H 2 O 2 in cell viability. |
Cytotoxicity was assessed by the activity of LDH released into the culture medium. LDH is a biochemical marker for the integrity of the membrane. LDH activity was measured using a kit for LDH. Secretion was measured LDH to the medium after 2.5, 6, and 24 h, the visible light irradiated in 3.6 and 12J / cm.
Also observe the
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● It found that the amount of LDH released into the medium at various time points after or light between 3.6J / cm 2 to 12J / cm 2 was similar to that of LDH released into the culture illuminated and no increase in the level of LDH during 24 h due to release natural |