Effect of visible light irradiation in the [ Ca 2 + ] |
To determine whether changes in intracellular calcium are mediated by a specific calcium channel was added to cardiac cells 10 µ M of verapamil before irradiation LEVL. Fluorescence was measured. We determined whether the light could directly induce increased intracellular [ Ca 2 +] even without exogenous photosensitizers. |
a c d b a Blank( no lighting)
b Lighting 1.5 min x 3.6
J / cm 2 c Lighting 5 min x 12 J / cm 2 d Lighting 5 min x 12 J / cm 2 + 200 u / mL catalase( degrades H 2 O 2)
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● The levels of [ Ca 2 + ] are dose dependent LEVL lighting.
● Catalase but decreases the amount of H 2 O 2 has a similar reaction against the [ Ca 2 + ].
● The Verapamil if it inhibits the increase in [ Ca 2 + ] but does so in a very low.
● NOTE, the curve b of the first figure and the second is at the same conditions.
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