E&E Magazine Volume 1 - 2013 | Page 67

TEST
MATERIALS
AND
METHODS
Measure ments of ROS
Measurements of superoxide anion radicals by electron paramagnetic spin resonance( EPR) using the spin DEPMPO which reacts with ROS as follows:
RESULTS
illuminated with 3,6 J / cm 2 a Control
c) DEPMPO with light
b) DEPMPO Sin iluminación
CONCLUSIONS
● The first figure indicates the LEVL illumination increases the concentration of H 2 O 2 in the cells.
● Resonance results following four figures show the lighting level
-­‐ increases LEVL O!
- in cardiomyocytes. And confirms the presence
-­‐ of O! d) DEPMPO superoxide dismutase with light