morphology of the cells 24h after illumination or treatment with H 2 O 2. The cultured cells are immunocytochemically stained for sarcomeric α- actin( red) to observe and contractile filaments against stained with hematoxylin( blue) to observe the core.
Control
12 µ M H 2O 2 Mild disorganized fibrils Decreased α-actin staining No change in the nucleus
24 µ M H 2O 2 Disorganization in fibrils cytoplasmic vacuolization Pyknotic damage in the nucleus 65 % good cells
exotoxins. |
● The |
toxic |
effect |
of |
visible light or H 2 O 2 to |
cardiomyocytes is dose |
dependent. |
An |
illumination |
below |
3.6J / cm 2 |
or |
concentrations |
below |
24 µ M H 2 O 2 cause no |
harm to most of the |
cells after 24 hours of |
treatment. |
Therefore, |
visible |
light |
illumination |
with |
energy less than 3.6 |
J / cm 2 |
appears safe for |
cardiomyocytes. |
48 µ M H 2O 2 Disorganization in fibrils Alteration staining structure cytoplasmic vacuolization Pyknotic damages the nucleus and perinuclear edema
3.6 J / cm 2 x 1.5 min No alteration
7.2 J / cm 2 x 3 min Decreased α-actin staining No change in the nucleus
12 J / cm 2 x 5 min Disruption of fibrils Decreased staining cytoplasmic vacuolation Damage oncotic( swelling of the cytoplasm)