advantages and disadvantages of gel filtration chromatography 7 | Page 3

Gives an estimate of molecular size in any practically any solution;
Precision is not so good.
Troubleshooting:
1. Poor resolution
Reason: Poor packed bed
Recommended solution: Check the efficiency of the column and re-pack if
necessary.
Always check the efficiency of a new column, especially one that has been
packed in the lab, before using it for critical applications.
Reason: Contamination of the bed with lipids, denatured protein etc.
Recommended solution: Thoroughly clean the column following the
recommended protocols given in the instructions provided by the column
manufacturer.
2. Poor yields
Reason: Contamination of the bed with lipids, denatured protein etc.
Recommended solution: Thoroughly clean the column following the
recommended protocols.
Tip: Running eluent slowly through the column can prevent microbial