advantages and disadvantages of gel filtration chromatography 7 | Page 2

There are three main applications of gel
filtration chromatography:
1. In group separations
Adjusting pH, buffer type, salt concentration
during sample preparation;
Removing interfering small molecules (EDTA,
Gu-HCl, etc);
Removing small reagent molecules (fluorescent
labels, radioactive markers, etc);
But not when the protein will precipitate.
2. In fractionation
Excellent during the polishing stage;
Removes dimers and other aggregates;
Transfers protein to buffer solution ready for
the next set of experiments;
Not so suitable if the sample volume is large.
3. For size estimation
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