advantages and disadvantages of gel filtration chromatography 7
Gel Filtration Chromatography
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Gel filtration, as known as size exclusion
chromatography (SEC), separates proteins
according to their different size as they pass
through a gel filtration column. Compared to
affinity chromatography or ion exchange
chromatography, proteins to be seperated by
gel filtration chromatography do not need to
bind to the chromatography medium, making
buffer composition hardly affect resolution. The
major advantages and disadvantages of gel
filtration chromatography are listed as below: