DIY
DO IT YOURSELF
10
Easy How-To Steps:
Label 2 eppendorf tubes:
+pGLO and -pGLO
Put 0.25mL of transformation solution (CaCl₂) into each, using a transfer pipette.
Place tubes on ice.
Use innoculation loop to pick up a bacteria colony
from starter plate. Immerse loop into +pGLO tube.
Place back on ice.
Sterilize loop by placing into a flame.
Repeat step 3 with -pGLO tube.
Put 10μL of plasmid DNA using a micropipette
ONLY INTO +pGLO