NTU Undergraduates' research April 2014 - Biosciences | Seite 73

FAYZAH ABDULLAH (N0329581)

CAMPYLOBACTER INTERACTION WITH PROBIOTIC ORGANISMS

ABSTRACT
The most common bacteria causing enterocolitis in human is Campylobacter jejune. Probiotics are
effective in inhibiting some enteric infection. The inhibition of Campylobacter is caused by lactic
acid which is produced by Lactobacilli. Many probiotic strains such as Lactic acid bacteria and
Bifidobacterium have been presented in dietary and pharmaceutical methods and established in
market for human ingestion for example probiotic capsule and yoghurt. The study was begun by
identifying the strains using morphological, physiological and biochemical methods. Two types of
Lactic acid bacteria species were used in this study which was cultured from five types of samples;
four from different yogurt brands and one sample from a probiotic capsule. The main objective of
this study was to define the ability of probiotic bacteria to inhibit the growth of Campylobacter.
This ability effect of inhibitory was determined by agar spot test and Cell free supernatant (CFU)
well agar diffusion method using four Campylobacter strains. These are Campylobacter jejuni
NCTC11168, RM1221, M1 and 01/51 which were tested with probiotic species from different
yogurt such as Yakut, Yeo, Strawberry Yoghurt, Actimel and Probiotic capsule. Probiotic species
tested were Lactobacillus acidophilus and Lactobacilli paracasei. It has been revealed in this
experiment that Lactic acid bacteria have slightly antimicrobial effect and no inhibitory effects
were obtained on cell free supernatant.