NTU Undergraduates' research April 2014 - Biosciences | страница 72

The effect of paraoxon and parathion on tissue transglutaminase activity
Yolanda Mason
School of Science and Technology, Nottingham Trent University, Nottingham NG11 8HJ

Abstract

The organophosphates paraoxon and parathion are highly toxic pesticides that are primarily
used in agriculture and as nerve agents. The present study was focused on the effect of
each concentration of the organophosphates compounds paraoxon and parathion on tissue
transglutaminase (TGase 2) activity using the biotin cadaverine incorporation assay.
Western blot analysis indicated the presence of TGase 2. Five concentrations of paraoxon
and parathion 3µl, 6µl, 12.5µl, 25µl and 50µl were compared with the untreated control to
measure TGase 2 activity. Paraoxon showed no significant effect on tissue tranglutaminase
activity. However, parathion demonstrated a significant decrease in TGase 2 activity. The
findings suggest that paraoxon has no effect on TGase 2 activity and parathion had an
inhibitory effect on TGase 2 activity.
Keywords: paraoxon, parathion, biotin cadaverine incorporation assay, tissue
transglutaminase (TGase 2) (tTG)