NTU Undergraduates' research April 2014 - Biosciences | Página 42

The effect of morin on H9c2 cell viability
Christina Tang

ABSTRACT
The flavonoid morin hydrate (3,5,7,2’,4’ pentahydroxyflavone) a polyphenol compound found in a
variety of different plant sources such as figs and osage oranges, have been reported to act as
potent antioxidants and are thought to be beneficial against the effects of oxidative stress and other
related diseases such as cancer, cardiovascular diseases and inflammatory conditions. However,
there have been reports of cytotoxicity in flavonoids such as naringenin, quercetin and taxifolin. This
study aims to determine the pro-oxidant properties of this moleculeat different dosagesand
exposure times. To determine its effects on both differentiated and undifferentiated embryonic ratderived H9c2 cells, as well as any cardio-protective effects of this flavonoid against H 2 O 2 induced
oxidative stress. We demonstrate, through the use of Tetrazolium Salt Thiazolyl (MTT) reduction
assay that morin hydrate does not appear to have significant pro-oxidant abilities and studies show a
generally cardio-protective quality against H 2 O 2 induced oxidative stress in this flavonoid, suggesting
an experimental anomaly. There also appears to be no significant difference in pro-oxidation
capabilities on differentiated and undifferentiated H9c2 cardiomyocytes.

KEY WORDS
Flavonoid; Antioxidant; Morin hydrate; Pro-oxidant, H9c2 cells, Cell viability