NTU Undergraduates' research April 2014 - Biosciences | Page 127

Isolation of Campylobacter jejuni from Free-range and Standard-range Retail
Poultry.
Ashley Jaggi BSc (Hons) Biomedical Science

School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS

Abstract: Campylobacter jejuni is considered one of the leading causes of human gastroenteritis
across the world. The prevalence of Campylobacter jejuni in retail poultry is thought to be one of
the major sources of infection to humans. There is some discrepancy within the published
literature as to whether there is a notable difference between free-range and standard-range
broilers in regards to Campylobacter jejuni colonisation. This study was conducted in order to
investigate this claim. A total of 10 chickens bought from a small range of UK supermarkets were
sampled and through conventional plating on mCCDA agar and enrichment techniques using
Preston Broth. Suspected Campylobacter spp. colonies were confirmed through gram staining,
oxidase testing and speciation PCR.
It was ultimately found that F