My first work Chang Liver cells Introduction | Page 2
thawing immediately after removing it, and gently shake the frozen tube to make it melt
completely within 1 minute, and pay attention that the water surface should not exceed the edge
of the frozen tube cover, otherwise it is prone to contamination. Then it was resuscitated and the
culture medium was changed the next day.The selective effects and mechanisms of Archaisms
275 on human gastric cancer cells sgc-7901, human gastric mucous normal cells ges-1 and Chang
liver/ Chang liver cells have been studied experimentally, providing theoretical basis for the
clinical application of Acidosis. Sgc-7901 cells, gs-1 and Chang liver/ Chang liver cells were
treated with ms-275 at different final concentrations, and the growth inhibition effect of the drug
on cells was detected by wst-1 method. Annexation and PI double standard flow optometry was
used to detect the selective killing effect of ms-275 on tumor cells sgc-7901 and normal
cells.TUNEL staining was used to observe the apotheosis in tumor cell nucleus.The results showed
that ms-275 significantly inhibited the growth of gastric cancer cell sgc-7901, and this effect was
time-dependent and dose-dependent. The killing effect of ms-275 was selective and showed no
apotheosis promoting effect on normal gs-1 cells and Chang liver human change's
phagocytes.TUNEL staining showed that ms-275 can induce DNA breakage in the nucleus of
sgc-7901 cells, which is an important indicator of cell apotheosis.
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