Chang Liver cells Introduction
Chang liver cells were established in 1954 from non - malignant human tissues.Widely used in
virology, biochemistry and malignant tumor related metastasis research.Chang liver/ Chang's
ATCC was determined to have He LA cell contamination as its origin through isomerism analysis,
He LA marker chromosome analysis, and DNA fingerprinting.Chang liver/ Chang liver cell
instructions:
1) for cells transported at room temperature, after receiving the cells, check whether there is a
transport problem, that is, whether the cell culture bottle or tube is damaged, whether the cell
culture fluid is overflowing.If there is no transportation problem, please disinfect with 75%
alcohol, keep the sealing film, and put it into the cell culture box for rest.Strictly check incubator
parameters: temperature, humidity and CO2 concentration.Generally, the stasis time of cells is
6-12 hours, and the subsequent operation can be started after the cells are in a stable state.With
the correct cell culture system, Chang liver/ Chang liver cells were cultured in strict accordance
with the instructions.When conditions permit, the cells were photographed within the first three
days of culture.
2) for the cells transported by dry ice, please put the frozen tube into a 37C tank for quick