Mi primera publicacion GRAM POSITIVOS (Revista Digital 2da Edición 2019) | Page 35

REVISTA GRAM POSITIVOS 2da. Edición.  
INTRODUCTION 
Moniliophthora   roreri   is   one   of   the   most   serious   issues   that   cacao   crop   has   in   Latin   American  
producer   countries.   There   are   losses   in   national   production   up   to   40   even   50%   of   the   harvest.  
Besides   there   are   chemical   products   in   the   market,   low   efficiencies   and   high   environmental  
impact   is   pointed   out.   Moreover,   old   crops   increase   the   issue   since   low   productivity   without   any  
resistance of the disease is detected. 
MATERIALS AND METHODS 
In   Colombia,   AGROSAVIA,   the   Colombian   Research   Centre   for   Agriculture,   is   a   decentralized  
non-profit   organization   granted   by   the   Colombian   Ministry   of   Agriculture   and   Rural  
Development,   to   tackle   issues   affecting   the   productivity   and   rentability   of   economic   interesting  
crops   in   the   country.   During   two   seasonal   experiments   between   2014-2016,   several   treatments  
where performed in order to pursue new technologies to defeat the disease. 
A   biological   treatment   consisted   on   the   amendment   of   Bacillus   amyloliquefaciens   Bs006   and  
Trichoderma   asperellum   Th406,   contrasting   to   M.   roreri   Mn004   all   these   strains,   belonging   to  
the   Colombian   Germplasm   Bank   of   Microorganisms   for   Agricultural   purposes,   were   selected  
and   tested   by   isothermal   calorimetry   (IMC)   using   a   TAM   Air   8   channels   equipment   (TA  
Instruments,   DE,   US).   The   strains   were   evaluated   against   the   phytopathogen   and   the   fractional  
inhibitory   concentration   index   (FIC)   was   calculated   using   the   heat-flow   as   an   analytical  
parameter. 
The   kinetical   growth   parameters   such   as   growth   rates,   maximal   growth   rates   and   lambda   were  
also calculated from the thermal monitoring. 
Qmax   was   compared   from   treatments   to   controls.   As   positive   control   was   used   Azoxystrobin  
(500   mg.kg-1).   As   negative   control   was   used   the   sole   growth   of   M.   roreri   Mn004.   All   treatments  
were performed per triplicates and the results were expressed as the average of. 
RESULTS AND DISCUSSION 
The   treatment   with   T.   asperellum   Th406   was   the   most   success   to   control   population   of   M.   roreri  
in   time   (after   40h   of   exposition).   The   growth   rate   was   higher   (11.47   h-1)   with   a   maximum   heat  
flow   of   3.95   and   a   Qmax   of   54.356   Joules.h-1.   Hence,   the   FIC   index   adding   Th406   was   4,88   which  
is an evidence of “Antagonic behaviour” according to the related scale. 

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