One Pot Biomass Pretreatment, Saccharification
and Fermentation
Lawrence Berkeley National Laboratory
Systems that advance market competitiveness of fuels and chemicals
produced from sustainable non-food crops
Following
are
representative
technologies
developed
at
the
Joint
BioEnergy
Institute
(JBEI)
to
simplify
biomass
pretreatment,
hydrolysis,
and
fermentation
including
new,
low-‐cost
ionic
liquids
to
enable
rapid
and
high-‐yielding
sugar
recovery
from
biomass.
Streamlined
Saccharification
from
Lignocellulosic
Biomass
(2015-‐143)
Blake
Simmons,
Seema
Singh,
and
Feng
Xu
have
developed
a
technology
using
bio-‐derived
ionic
liquids
in
high
biomass-‐loading
pretreatment
followed
by
fed
batch
saccharification
to
yield
a
sugar
stream
that
can
be
used
instantly
for
microbial
fermentation,
without
additional
steps.
The
technology
reduces
the
use
of
ionic
liquids
and
water
significantly
and
diminishes
wastewater
generation
to
lower
the
cost
of
cellulosic
ethanol
production
overall.
Bio-‐derived
ionic
liquids
are
also
known
for
their
low
toxicity
to
hydrolytic
enzyme
mixtures
and
microbes.
Consolidated
Conversion
of
Biomass
into
Biofuels
using
Ionic
Liquids
(2014-‐139)
By
overcoming
the
pH
mismatch
between
pretreatment
and
downstream
unit
operations
with
cholinium-‐based
ionic
liquids
using
commercially
available
enzyme
mixtures
and
fermentation
hosts,
this
technology
eliminates
the
requirement
for
extensive
rinsing
of
the
pretreated
biomass
and
/
or
post-‐treatment
for
substantial
savings
in
energy
and
water
consumption.
Switchable
Ionic
Liquids
for
Biomass
Pretreatment
and
Enzymatic
Hydrolysis
(2014-‐064)
This
one-‐pot
technology
enables
both
efficient
pretreatment
of
biomass
and
saccharification
of
cellulose
without
removing
the
ionic
liquid
used
in
the
process
by
taking
advantage
of
the
ionization
states
of
carboxylic
acids
to
switch
from
a
basic
solution
that
pretreats
biomass
to
an
acidic
solution
with
conditions
favorable
for
enzymes
that
break
down
polysaccharide
biomass.
The
technology
enables
efficient
recovery
and
recycling
of
the
ionic
liquid
to
avoid
the
expense
of
purchasing
new
ionic
liquids
for
each
pretreatment
run.
JBEI
is
an
LBNL-‐led
research
partnership
of
Lawrence
Berkeley
National
Laboratory,
Lawrence
Livermore
National
Laboratory,
Pacific
Northwest
National
Laboratory,
Sandia
National
Laboratory,
the
University
of
California,
Berkeley,
the
University
of
California,
Davis,
and
the
Carnegie
Institution
for
Science.
For
information
on
partnership
opportunities:
Peter
Matlock,
JBEI
Director
of
Commerci alization
[email protected],
510-‐486-‐4803
ipo.lbl.gov
jbei.org