Lab Matters Winter 2023 | Page 15

INDUSTRY MATTERS environmental sample , at much fewer reads than DNA and RNA shotgun workflows . Combined with optimized concentration and extraction methods , NGS delivers efficient , comprehensive genomic coverage compared to quantitative PCR methods . As bottlenecks to routine implementation of wastewater-based surveillance are addressed via technical advances , crosssector collaborations , data standards development , and sustained funding and policy support , there is untapped potential for NGS to provide an improved understanding of pathogen circulation at the population level . g
Illumina is a Platinum Level Sustaining Member of APHL .
References
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2 . Hewitt J , Trowsdale S , Armstrong BA , Chapman JR , Carter KM , Croucher DM , Trent CR , Sim RE , Gilpin BJ . Sensitivity of wastewater-based epidemiology for detection of SARS-CoV-2 RNA in a low prevalence setting . Water Res . 2022 Mar 1 ; 211:118032 . doi : 10.1016 / j . watres . 2021.118032 . Epub 2022 Jan 2 . PMID : 35042077 ; PMCID : PMC8720482 .
3 . Kirby AE , Welsh RM , Marsh ZA , et al . Notes from the Field : Early Evidence of the SARS-CoV-2 B . 1.1.529 ( Omicron ) Variant in Community Wastewater — United States , November – December 2021 . MMWR Morb Mortal Wkly Rep 2022 ; 71:103 – 105 . DOI : http :// dx . doi . org / 10.15585 / mmwr . mm7103a5external icon .
4 . Kotlarz N , Holcomb DA , Tanvir Pasha ABM , Reckling S , Kays J , Lai YC , Daly S , Palani S , Bailey E , Guidry VT , Christensen A , Berkowitz S , Hoppin JA , Mitasova H , Engel LS , Reyes FLL 3rd , Harris A . Timing and Trends for Municipal Wastewater , Lab-Confirmed Case , and Syndromic Case Surveillance of COVID-19 in Raleigh , North Carolina . Am J Public Health . 2023 Jan ; 113 ( 1 ): 79-88 . doi : 10.2105 / AJPH . 2022.307108 . Epub 2022 Nov 10 . PMID : 36356280 ; PMCID : PMC9755929 .
5 . Nyaruaba R , Mwaliko C , Dobnik D , Neužil P , Amoth P , Mwau M , Yu J , Yang H , Wei H . Digital PCR Applications in the SARS-CoV-2 / COVID-19 Era : a Roadmap for Future Outbreaks . Clin Microbiol Rev . 2022 Sep 21 ; 35 ( 3 ): e0016821 . doi : 10.1128 / cmr . 00168-21 . Epub 2022 Mar 8 . Erratum in : Clin Microbiol Rev . 2023 Jun 21 ; 36 ( 2 ): e0005223 . PMID : 35258315 ; PMCID : PMC9491181 .
6 . Varkila MRJ , Montez-Rath ME , Salomon JA , Yu X , Block GA , Owens DK , Chertow GM , Parsonnet J , Anand S . Use of Wastewater Metrics to Track COVID-19 in the US . JAMA Netw Open . 2023 Jul 3 ; 6 ( 7 ): e2325591 . doi : 10.1001 / jamanetworkopen . 2023.25591 . PMID : 37494040 ; PMCID : PMC10372707 .
7 . Zhou Y , Zhang L , Xie YH , Wu J . Advancements in detection of SARS-CoV-2 infection for confronting COVID-19 pandemics . Lab Invest . 2022 Jan ; 102 ( 1 ): 4-13 . doi : 10.1038 / s41374-021- 00663-w . Epub 2021 Sep 8 . PMID : 34497366 ; PMCID : PMC8424153 .
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Winter 2023 LAB MATTERS 13