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Effect of different levels of exercise on telomere length includes breathing exercises and meditation, was also found to be positively associated with telomere length. Breathing exercises and meditative components have been shown to have positive effects on psychological health, including alleviation of stress, anxiety and depression, which are significantly associated with shortened telomere length (50, 51). In addition, in subgroup analysis according to age, the current study found that exercise had a positive effect on leukocyte telomere length in younger individuals, but not in elderly subjects. Some researchers found that exercise did not positively affect leukocyte telomere length in a group of young subjects (33, 38). However, another study involving healthy adolescents found that exercise resulted in increased leukocyte telomere length (51). This discrepancy may be attributed to differences in sample size and methods of telomere measurement and, perhaps, selection bias in these studies. The current study has several limitations that should be addressed. First, the strength of evidence may be weakened due to deficiency of data in some studies, and differences in tissue sources, sample size and methods of evaluation of telomeres. Secondly, it was not possible to perform formal subgroup analysis ac- cording to age, except for the elderly group, because age varied widely across the included studies. Thus, the current study could not provide sufficient evidence to support the association between exercise and telomere length according to age. Finally, the pooled synthesis of observational studies in this meta-analysis may have limited the quality of evidence compared with larger, randomized controlled trials. Conclusion Compared with inactive individuals, active subjects had longer telomere lengths, especially those who engaged in moderate and robust physical exercise. Further prospective, large, randomized controlled studies are needed to determine the effect of different types of physical activity on telomere length. ACKNOWLEDGEMENTS The authors thank the staff of the National Clinical Research Center of Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China. The authors have no conflicts of interest to declare. REFERENCES 1. Yao Y, Dai W. Genomic instability and cancer. J Carcinogen Mutagen 2014; 5: 1000165. 2. Blackburn EH. Switching and signaling at the telomere. Cell 2001; 106: 661– 673. 477 3. Lopez-Otin C, Blasco MA, Partridge L, Serrano M, Kroemer G. The hallmarks of aging. Cell 2013; 153: 1194–1217. 4. Prasad KN, Wu M, Bondy SC. Telomere shortening during aging: attenuation by antioxidants and anti-inflammatory agents. 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