What’s Going On - In the Classroom
PRINCIPLES OF ENGINEERING
Mr. Kahler
Students built a one-string guitar and learned basic component circuitry. They were trained on how to solder
and are currently building an operational amplifier for their guitar. Next week they’ll be building functioning
speakers.
PLTW MEDICAL INTERVENTIONS &
BIOMEDICAL INNOVATIONS
Mr. Arnholt
PLTW has been ending the year with some fantastic labs on bio-engineering.
Medical interventions used columns to separate out different proteins
produced by E.coli. The protein of interest is GFP, a glowing florescent protein
that they made the bacteria produce through a bacterial transformation. They
will be doing analysis of these products with a vertical gel electrophoresis
to look at their molecular weights to determine the protein sizes.
In Biomedical innovation, students took a gene (Kan r) and put it
into a ring of DNA called a plasmid. The gel shown shows all of the
different ways this gene could be inserted into the plasmid and the
conformational differences between the products. Next, students will
get bacteria to use the plasmid to give them antibiotic resistance.
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