Garuda Indonesia Colours Magazine April 2017 | Page 26
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Explore | Masterclass
The image from
a microscope has a very
shallow focus that makes
seeing difficult when
first getting oriented.
When I first look into a microscope, things
have no frame of reference. The image from
a microscope has a very shallow focus that
makes seeing difficult when first getting
oriented. Many subjects are invisible because
of low contrast, or are transparent, making
them very hard to find. Some sample
characteristics are visible only when using
highly specialised techniques. These are
a few of the problems I face when
photographing the invisible.
Saat saya pertama kali melihat ke dalam
mikroskop, objek di dalamnya tidak memiliki
kerangka acuan. Gambar dari mikroskop
memiliki fokus yang sangat dangkal sehingga
sulit ditangkap mata. Banyak objek tak
terlihat karena kontras yang rendah,
atau transparan, sehingga sangat sulit
ditemukan. Beberapa sampel hanya bisa
dilihat menggunakan teknik khusus. Inilah
beberapa tantangan yang saya hadapi ketika
memotret objek yang tak terlihat.
Pine Stem Panorama
This picture features a cross-section of Pinus or pine stem.
It was photographed using a low-power objective. Four pictures
were made of the sample and stitched into this composite file.
The sample was illuminated using darkfield illumination.
I think the sample almost looks astronomical. Visible in the
photomicrograph is the old wood (pith) in the middle, the growing
wood (xylem) outside of the pith, responsible for water transport,
and the phloem, which transports the simple sugar produced from
photosynthesis. The outermost layer is called epidermis or bark.
The stem was 5mm in diameter, and the microscope produced
a magnification of 25 times.
Objek dalam foto ini adalah penampang atau irisan melintang
batang pinus. Foto ini dihasilkan menggunakan lensa objektif
mikroskop berdaya rendah. Empat foto dari sampel digabungkan
menjadi satu berkas komposit. Sampel diterangi dengan teknik
pencahayaan dark field. Saya pikir sampel ini jadi terlihat astronomis.
Fotomikrograf ini memperlihatkan kayu tua (empulur) di tengah,
pembuluh kayu (xilem) di luar empulur, yang berfungsi mengangkut
air, dan pembuluh tapis (floem), yang mengangkut gula hasil dari
fotosintesis. Lapisan terluar disebut epidermis atau kulit kayu.
Batang pinus ini memiliki diameter 5 mm, dan mikroskop
menghasilkan perbesaran 25 kali.