Linear Plots of the Michaelis–Menten Equation
The Lineweaver–Burk plot or double reciprocal plot is a common way of
illustrating kinetic data. This is produced by taking the reciprocal of both sides
of the Michaelis–Menten equation.
As shown on the right, this is a linear Naturally, no experimental values can be
form of the Michaelis–Menten taken at negative 1/[S]; the lower limiting
equation and produces a straight line value 1/[S] = 0 (the y-intercept)
with the equation y = mx + c with a y- corresponds to an infinite substrate
intercept equivalent to 1/Vmax and an concentration, where 1/v=1/Vmax as
x-intercept of the graph representing shown at the right; thus, the x-intercept
−1/Km. is an extrapolation of the experimental
data taken at positive concentrations.
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