Michaelis–Menten Kinetics
Michaelis–Menten equation: ν0 = d[P]/dt = Vmax[S]/(Km+[S])
• v0 indicates the reaction rate and the activity of the enzyme;
• [S] represents substrate concentration;
• Vmax indicates maximum reaction rate;
• Km is Michaelis constant, Km= (K-1+K2) / k1.
The value of Michaelis constant is equal to the substrate concentration
when the enzyme reaction rate is half of the maximum reaction rate.
Km represents the affinity of the enzyme to the substrate. A high Km
value indicates a weak affinity between E and S, and a low km value
indicates a strong affinity.
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