ANALYSIS OF HUMAN MESENCHYMAL STEM CELL METABOLIC HETEROGENEITY
DURING EXPANSION
Yijun Liu1, Nathalie Muñoz2, Teng Ma1, Timothy M. Logan2
1
Department of Chemical and Biomedical Engineering, Florida State University, 2525 Pottsdamer
Street, Tallahassee, FL 32310 USA
2
Department of Chemistry and Biochemistry,Florida State University Tallahassee, FL 32306
Human mesenchymal stem cells (hMSCs) are a primary cell source in cell therapy for a wide range of
diseases. However, bone marrow derived hMSCs are intrinsically heterogeneous and comprise
subpopulations that differ in their proliferation, multi-potency, and functional properties. The
conventional cell culture techniques used in producing hMSC for clinical studies lead to a gradual loss of
the multipotent subset and cellular senescence. Understanding hMSC metabolism is important in
designing an approachable strategy for hMSC expansion while maintaining their therapeutic potency. In
this study, a GC-MS-based Y]X