Page 23
Aurora
Crystal Violet
Sodium Chloride
pH indicator
Agar
Procedure:
1. Suspend the measured amount of powder in 1 liter of purified water and mix thoroughly+
2. Heat with frequent steering and boil for 1 minute to completely dissolve the powder+
3. Autoclave the prepared medium at 121°C for 15 minutes in order to sterilize+
Mannitol salt agar
This is a selective medium used for the isolation of staphylococci+ The medium consists of digests of casein and
animal tissue, beef extract, mannitol, salts, and phenol
red+ Staphylococci can grow in the presence of a high
sa