7 cells were exposed to paclitaxel( Taxol) or docetaxel( Taxotere) preparations as 6X, 5C and 15C potencies prepared from pharmacological concentration of 25 nmol / L, for 72 hours. The cell culture groups were evaluated with the trypan blue dye exclusion method for proliferation / cytotoxicity rates, immunostaining β-tubulin for microtubule organisation, and reverse transcription polymerase chain reaction for gene expression levels. Fold-change in gene expression was determined by the ΔΔCt method. The administration of the preparations had little or no cytotoxic effect on MCF-7 cells, but altered the expression of genes analysed with a complex effect. According to the ΔΔCt method with a five-fold expression difference( p < 0.05) as a cut-off level, preparations of paclitaxel and docetaxel showed differential effects on the studied genes with a concentration-independent activity. Furthermore, the preparations disrupted the microtubule structure of MCF-7 cells, suggesting that they retain their biological activity. In conclusion, the team was able to demonstrate that gene expression alterations occur after exposure to homeopathic preparations of taxane drugs.
2. Kishore L, Singh R. Effect of Cephalandra indica against advanced glycation end products, sorbitol accumulation and aldose reductase activity in homoeopathic formulation. Indian Journal of Research in Homoeopathy. 2018; 12( 1): 11-19.
This study was designed to evaluate the potential of homoeopathic preparations of Cephalandra indica L. against oxidative stress. The antioxidant activity of Cephalandra indica( mother tincture, 6C and 30C) was evaluated by employing various in-vitro antioxidant methods. The mother tincture, 6C and 30C of Cephalandra indica were found to have strong reducing power, 2,2-diphenyl-1-picrylhydrazyl radical, hydrogen peroxide, nitric oxide and superoxide radical scavenging activity. The percentage inhibition of advanced glycation end product formation by mother tincture, 6C and 30C of Cephalandra indica( 10 – 50 μl) was found to be 30.34 %– 91.77 %, 29.98 %– 65.71 % and 33.05 %– 57.75 %, respectively. Mother tincture, 6C and 30C of Cephalandra indica showed an inhibitory effect against sorbitol accumulation with IC50 value of 26.12 μl, 203.10 μl and 897.3 μl, respectively, whereas, in aldose reductase inhibition assay, the IC50 value was 32.54 μl, 175.02 μl and 834.34 μl, respectively. The results revealed that homoeopathic preparations of Cephalandra indica exhibit a protective effect against oxidative stress.
3. Dalboni LC, et al. Biological Actions, Electrical Conductance and Silicon-Containing Microparticles of Arsenicum Album Prepared in Plastic and Glass Vials. Homeopathy. 2019; 108( 1): 12-23. This research was carried out to determine if silica is present in homeopathic medicines produced in glass or plastic vials, if it had any effect on the activity of the homeopathic medicine contained therein, and what effect, if any, homeopathically prepared Arsenicum album had on macrophage activity. To do this, the research team compared the biological activity, electrical current and silicon microparticle content( determined by means of scanning electron microscopy / energy-dispersive X-ray spectroscopy) of high dilutions( HDs) of arsenic prepared in plastic and glass vials, to investigate the role of silica in their biological effects in-vitro. Co-cultures of macrophages and yeast( Saccharomyces cerevisiae) were treated with different HDs of arsenic prepared in plastic and glass vials. Macrophage morphology, phagocytosis index, nitric oxide( NO), and cytokine production were evaluated. Measurable amounts of silicon microparticles were detected only in the HDs prepared in glass vials, but ultra-centrifugation eliminated them. Specific and non-specific results were observed. Non-specific pro-inflammatory effects were seen in all dilutions prepared in plastic vials, including elevation of pro-inflammatory cytokines, NO and macrophage phagocytic index. Only the 200C potency of arsenic produced a specific decrease in interleukin-6 production in macrophages, and it was independent of the vial type or the presence of microparticles of silica in the medicine samples. In addition, the nature of the vials had an impact on the electric flow in the respective fluids.
NEW 1:1 Glycetract Range Launched in May 2025
Our point of difference:- 7 % Fulvic Minerals in every 500ml Significant savings on cost Free from chemical preservatives
www. bioactiv. com. au over 30 now available
Bitter Melon 1:1( Momordica Charantia) 500ml Cinnamon 1:1( Cinnomomum Cassia) 500ml Cistanche 1:1( Cistanche Deserticola) 500ml Codonopsis 1:1( Codonopsis Pilosula) 500ml Corydalis 1:1( Corydalis Capnoides) 500ml Damiana 1:1( Turnera Diffusa) 500ml Devil ' s Claw 1:1( Harpagophytum Procumbens) 500ml Echinacea 1:1( EchinaceaPurpurea( Linn.) Moench) 500ml Eclipta Alba 1:1( Eclipta Prostrata) 500ml ElderberryFlower 1:1( SambucusWilliamsiiHance) 500ml Fennel Seed 1:1( Foeniculum vulgare) 500ml Polyogonium Multiform( Fo-Tienc) 1:1 500ml Globe Artichoke 1:1( Cynara Scolymus) 500ml Graviola 1:1( Annona Muricata) 500ml Hawthorn Berry 1:1( Crataegus Monogyna) 500ml Horny Goat Weed 1:1( Epimedium Brevicornum) 500ml Jamaican Dogwood 1:1( Cornus Officinalis) 500ml Lavender 1:1( Lavandula Angustifolia) 500ml Meadowsweet 1:1( Spiraea Salicifolia) 500ml Mistletoe 1:1( Viscum Album) 500ml Mullein 1:1( Verbascum Thapsus) 500ml Olive Leaf 1:1( Olea Europaea) 500ml Paeonia( Peony) 1:1( Paeonia Lactiflora) 500ml Panax Pseudo Ginseng 1:1( Panax Notoginseng) 500ml Rehmannia 1:1( Rehmannia Glutinosa) 500ml Rhodiola Rosea 1:1( Rhodiola Rosea) 500ml Sarsaparilla 1:1( Smilax Officinalis) 500nl Siberian Ginseng 1:1 500ml Tienchi Ginseng 1:1( Panax Notoginseng) 500ml Tongkat Ali 1:1( Eurycoma Longifolia) 500ml Tribulus 1:1( Tribulus Terrestris) 500ml Nettle Leaf 1:1( Urtica Dioica) 500ml
94 | vol31 | no2 | JATMS