homeopathic management of autism . A total of 60 children of both sexes diagnosed with autism aged ≤12 years were selected . A non-randomised , self‐controlled , pre- and post‐intervention study design was used . An initial 6 month observation period was employed as the control period and the same patients were treated for 1 year . Outcome changes were measured using the Autism Treatment Evaluation Checklist and the Autistic Hyperactivity Scale . The study demonstrated a significant reduction of hyperactivity , behavioural dysfunction , sensory impairment and communication difficulty . The medicines most commonly used were Stramonium , Tarentula hispanica , Calcarea carbonica , Natrum muriaticum , and Carcinosinum .
In-Vitro Studies
1 . Chikramane PS , et al . Metal nanoparticle induced hormetic activation : A novel mechanism of homeopathic medicines . Homeopathy . 2017 ; 106 ( 3 ): 135-44 . The team conducting this work sought to assess the effects of homeopathically potentised metals on cultured cells to answer the question of whether such negligible metal concentrations elicit a biological response . The effects of metal-based homeopathic medicines ( 30C and 200C ) were analysed at doses between 0.003 % v / v and 10 % v / v in in-vitro HepG2 cell-line . Upon treatment , cell response was estimated by MTT assay , FACS and total intracellular protein . Experiments were performed to discern whether the hormesis was a cellactivation or a proliferation effect . Remedies at doses containing a few femtograms / ml levels of the starting metals induced a proliferation-independent hormetic activation by increasing the intracellular protein synthesis . The metal concentrations ( at fg / ml ) were a billion-fold lower than the studies with synthetic NPs ( at μg / ml ).
2 . Khuda-Buksh AR , Mondal J , Shah R . Therapeutic potential of HIV nosode 30c as evaluated in A549 lung cancer cells . Homeopathy . 2017 ; 106 ( 4 ): 203-13 . This research set out to determine if a homeopathically prepared HIV nosode in 30C potency ( HIV 30C ) has therapeutic potential against lung cancer cells ( A549 ) as compared to the effects of the nosode against WRL-68 ( normal ) cells . The effects of HIV 30C were thoroughly tested for its possible anti-cancer potential on A549 cells ( lung cancer ); WRL-68 normal liver cells served as control . Three doses , one at LD50 and two below LD-50 , were used . Proliferation , migration and senescence assays were made , and the generation of reactive oxygen species ( ROS ) was studied by routine techniques . The ability of HIV 30C to induce apoptosis in A549 cells and its possible signalling pathway were determined using immunoblots of relevant signal proteins and confocal microscopy , including studies on telomerase reverse transcriptase ( TERT ) and topoisomerase II ( Top II ) activities , intimately associated with cell division and DNA replication . The results showed that HIV 30C prevented cancer cell proliferation and migration , induced pre-mature senescence , enhanced pro-apoptotic signal proteins like p53 , bax , cytochrome c , caspase-3 and inhibited anti-apoptotic signal proteins Bcl2 , TERT and Top II , changed mitochondrial membrane potential and caused externalization of phosphatidyl serine . It also induced apoptosis as evidenced from an increase in the numbers of cells with distorted membrane morphology , nuclear condensation , DNA fragmentation , and ROS , which is typical of apoptosis in progress .
3 . Gupta G , et al . Anti‐candidal activity of homoeopathic drugs : An in‐vitro evaluation . Indian J Res Homeopathy . 2015 ; 9:79-85 . In this study , samples collected from the oral cavities and tongues of patients suspected of suffering from oral candidiasis , were incubated for growth of Candida . Fermentation and assimilation tests confirmed the species as Candida albicans . The disc method was used to assess the in-vitro anti-candidal effect of several homeopathic drugs in 30C and 200C potencies against the fungi in in-vitro conditions and compared with standard antifungal drug ketoconazole ( control ), rectified spirit ( control / vehicle ) and distilled water ( vehicle ) by “ inhibition zone technique ”. The homeopathic drugs Benzoicum acidum , Apis mellifica , Kali iodatum , Mezereum , Petroleum , Sulphur , Tellurium , Sulphur iodatum , Graphites , Sepia , Silicea and Thuja occidentalis in 30C and 200C potencies were tested against Candida albicans . Mezereum in 30C and 200C potencies showed maximum inhibition of growth of Candida albicans followed by Kali iodatum 200C while Kali iodatum 30C and Petroleum 30C produced minimum inhibition .
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