RECENT RESEARCH evaluate the potential for inhalation of Longevity™ , a proprietary essential oil blend manufactured by Young Living Essential Oils ( Lehi , Utah , USA ), on energy levels among female survivors of COVID-19 who continue to experience fatigue more than 5 months recovery from the acute infection . Forty women were randomized to two groups : intervention and placebo . Both groups inhaled the assigned product twice daily for fourteen consecutive days . Fatigue scores were measured using the Multidimensional Fatigue Symptom Inventory ( MFSI ). Secondary outcomes included scores on each of the MFSI ' s ten subscales .
Results : Individuals who inhaled the essential oil blend for 2 weeks had significantly lower fatigue scores after controlling for baseline scores , employment status , BMI , olfactory function , and time since diagnosis , with a large effect size ( F ( 1,39 ) = 6.15 , p = . 020 , partial eta squared = 0.198 ). Subscale analysis identified subscales of vigor , as well as global , behavioral , general , and mental fatigue as benefiting from the intervention . This study provides evidence that a proprietary aromatherapy blend can significantly improve energy levels among women who are experiencing fatigue after recovering from COVID-19 .
Sinha M , Chakraborty U , Kool A , Chakravarti M , Das S , Ghosh S , Thakur L , Khuranna A , Nayak D , Basu B , Kar S , Ray R , Das S . In-vitro antiviral action of Eupatorium perfoliatum against dengue virus infection : Modulation of mTOR signaling and autophagy . J Ethnopharmacol . 2022 . doi : 10.1016 / j . jep . 2021.114627
Ethnopharmacological relevance : Dengue virus ( DENV ) is a re-emerging mosquito-borne flavivirus that has recently engendered large epidemics around the world . Consequently , antivirals with effective anti-DENV therapeutic activity are urgently required . In the 18th century , Europeans , as well as native inhabitants of North America , were known to adapt the medicinal property of the common perennial plant Eupatorium perfoliatum L . to treat fever and infections . Previous studies have shown that Eupatorium perfoliatum L . possesses anti-inflammatory , anti-oxidative , antiplasmodial , anti-bacterial and antiviral activities . However , to the best of our knowledge , no anti-DENV activity of E . perfoliatum L . has been investigated at the molecular level so far .
Aim of study : Here , for the first time we have attempted to study the action of E . perfoliatum extract and its few bioactive components i . e ., quercetin , caffeic acid and eupafolin against wild primary clinical isolate of DENV-2 infection in an in vitro model .
Materials and methods : The presence of the bioactive components in the E . perfoliatum extract , were analyzed by HPLC- DAD . Then , CC50 as well as IC50 values of the extract and its bioactive components were measured against DENV in HepG2 cell line . After that , the antiviral activity was studied by Time of addition assay using qRT-PCR . Further , the downstream signalling action of E . perfoliatum extract , was studied by Human phosphorylation MAPK antibody array , followed by immunofluorescence microscopy . Moreover , a molecular docking analysis was done to study the binding affinity of bioactive components of E . perfoliatum extract with TIM-1 transmembrane receptor protein , which is known for viral internalization .
Result : We found that E . perfoliatum extract has marked antiviral activity during pre-treatment against DENV infection in HepG2 cell line . The extract also significantly reduced the DENV induced autophagy in HepG2 cell line as detected by LC3 II localization . The presence of different bioactive compounds in E . perfoliatum extract were confirmed by HPLC-DAD . In the bioactive components , in parallel to earlier studies , quercetin showed the most significant preventive action against DENV infection . Further , in molecular docking analysis also , quercetin showed the strongest binding affinity towards DENV membrane receptor TIM-1 protein .
Conclusion : Our findings suggests that E . perfoliatum extract has significant potential to be an anti-DENV therapeutic agent . Moreover , among the bioactive components , quercetin may have a prophylaxis role in executing the antiviral activity of E . perfoliatum extract against DENV infection .
Ayurveda
Gundeti MS , Bhurke LW , Mundada PS , Murudkar S , Surve A , Sharma R , Mata S , Rana R , Singhal R , Vyas N , Khanduri S , Sharma BS , Srikanth N , Dhiman KS . AYUSH 64 , a polyherbal Ayurvedic formulation in Influenzalike illness - Results of a pilot study . J Ayurveda Integr Med . 2022 ; 13 ( 1 ): 100325 . doi : 10.1016 / j . jaim . 2020.05.010 . Epub 2020 May 14 .
Background : Influenza-like Illness ( ILI ) refers to a wide range of viral infections with an important cause of morbidity and mortality worldwide . The global incidence of ILI is estimated at 5-10 % in adults and 20-30 % in children . In India influenza accounts for 20-42 % of monthly acute medical illness hospitalizations during the peak rainy season . AYUSH-64 , a poly-herbal drug , is in practice for 40 years for various clinical conditions like fevers , microfilaremia , and inflammatory conditions .
Objective : A pilot study was conducted to evaluate the safety and efficacy of Ayurvedic formulation , AYUSH-64 in clinically diagnosed ILI for accelerating the recovery .
Material and methods : A prospective , open-label , nonrandomized , single group , single-center pilot clinical study with pretest and post-test design was conducted at Raja Ramdeo Anandilal Podar Central Ayurveda Research Institute for Cancer , Mumbai , an institute of Central Council for Research in Ayurvedic Sciences ( CCRAS ) between June 2018 and July 2019 . A total of 38 participants of clinically diagnosed ILI ( 18-65 years ) were studied with an one-week intervention of ' AYUSH 64 ' in a dose of 3 gm / day and three weeks post-treatment observation period . Assessment of parameters viz . improvement in the symptoms of ILI , frequency of usage of acetaminophen , antihistaminic and cough syrup , hematology , liver function and kidney
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