was assessed by quantification of apoptosis and necrosis using acridine orange / ethidium bromide . An analysis of the results showed that Canova significantly reduced DNA damage induced by NMU and significantly reduced the frequency of NMU-induced apoptosis after 24 hours of treatment .
Plant Studies
1 . Dinelli G , et al . Different Approaches in Homeopathic Basic Research : Plant-Based Bioassays and Evaporation-Induced Crystallization . Homeopathy , 2016 , 105 , 12 . Italian researchers from the University of Bologna carried this work out to confirm the effects of homeopathically prepared Arsenic in the form of As2O3 45X on the growth of wheat seedlings . The results demonstrated a significant increase of germination rate and stalk growth with respect to control . Further work was done using this model to determine the effects of temperature , the time taken for the germination effects to become apparent , succussion number and potency level , as well as gene expression , on the wheat-germination effects of As2O3 . From these experiments it was shown that As2O3 45X heated to 20 degrees C , 40 degrees C and 70 degrees C , induced a significant increase of germination rate vs . control , losing its effectiveness at 100 degrees C . A2O3 45x induced a stimulating effect on germination that reached significance only after three months from beginning treatment . In regard to the number of succussions used to prepare the As2O3 , a significant increase of germination was obtained starting from 32 succussions between each dilution step for As 45x . Homeopathic potencies from 5X to 60X were prepared and the data showed an oscillatory trend , with some potencies inducing a significant decrease ( 35X ), while others a significant increase of germination rate ( 5X , 30X , 40X , 45X , 55X , 60X ). In regard to gene expression , a massive reduction of gene expression levels to values comparable to those of the control group , induced by As2O3 45x , was observed for several functional classes of genes . In an extension to this , the research team sought to verify whether the droplet evaporation method ( DEM ) for crystal formation can be applied to assess the effectiveness of homeopathic medicines . They studied the shape characteristics of the polycrystalline structures formed during droplet evaporation of wheat seed leakages . The results showed that As2O3 45x increased the local connected fractal dimension levels and bilateral symmetry exactness values in the polycrystalline structures , as compared to the water treatment .
JATMS | Spring 2022 | 147