Walking On Volume 5, Issue 11, Nov. / Dec. 2018 | Page 8

For the Health of It An Improved Map of the Horse Genetic Code By University of Louisville Researchers fill gaps in equine reference genome to guide new approaches in fighting disease Research led by scientists at the University of Louisville and the University of Kentucky has pro- duced a more complete picture of the domestic horse reference ge- nome, a map researchers will use to determine the role inherited genes and other regions of DNA play in many horse diseases and traits important in equine science and management. By re-analyzing DNA from a thoroughbred named Twilight, the basis for the original horse refer- ence genome, scientists generated a more than ten-fold increase in data and types of data to correct thousands of errors in the origi- nal sequence that was released in 2009. Since then, there have been dramatic improvements in nucle- otide sequencing technology and the computational hardware and algorithms used to analyze data. It is now easier and less expensive to build a reference genome. The new equine reference ge- nome, known as EquCab3.0, was published today in Communica- tions Biology, representing the work of 21 co-authors from 14 universities and academic centers around the world. The horse refer- ence genome is publicly available through the National Center for Biotechnology Information, a di- vision of the National Institutes of Health. 8 • Walking On Genome sequencing allows researchers to read and decipher genetic information found in DNA and is especially important in map- ping disease genes – discovering diseases a horse might be genetical- ly predisposed to developing. Data gathered from future genetic and genomic studies of horses will use the new reference as a basis, which also has implica- tions for tackling serious diseases in humans, said principal investi- gator Ted Kalbfleisch, Ph.D., of the Department of Biochemistry and Molecular Genetics at the UofL School of Medicine. “Because we can sequence a horse and map it to the reference genome, we can know what genes might be affected by a mutation and come up with a hypothesis for what went wrong,” Kalbfleisch said. “Looking beyond the horse, we all want to cure cancer and other dis- eases that affect humans. Being able to accurately generate reference genomes gives us the tool that we need to map an individual’s genom- ic content. Having a high-quality reference genome makes it possible for us to know where an individu- al has a mutation and personalize therapies that will be right for an individual and the specific disease they have.” Senior author James MacLeod, V.M.D., Ph.D., of the University of Kentucky’s Gluck Equine Research Center added, “Increased accura- cy of the horse reference genome achieved through this work will greatly facilitate additional research in many aspects of equine science.  Medical advances for horses as a patient population, both in terms of sensitive diagnostic tests and emer- gent areas of precision medicine, are addressing critical issues for the health and wellbeing of these wonderful animals.” Financial support for the re- search was provided by the Morris Animal Foundation, the United States Department of Agriculture and several additional grants to the laboratories of individual co-au- thors.