Unequivocal Discrimination of Leucine and Isoleucine in De Novo Antib Unequivocal Discrimination of Leucine and Isoleuci | Page 7

Traditional Methods - Traditional
Mass Spectrometry
Limitations
It can only measure known amino acids/proteins.
It cannot distinguish between Leucine and Isoleucine.
If sequencing is incomplete or the mass spectrum lacks
matching of certain peptides or mutations, unknown
modifications may result in erroneous results.