Unequivocal Discrimination of Leucine and Isoleucine in De Novo Antib Unequivocal Discrimination of Leucine and Isoleuci | Page 5

Traditional Methods –
Edman Degradation
Limitations
It’s ineligible for the N-terminal blocked proteins, of
which the alpha-amino is modified with acetylation,
methylation, pyroglutamidation, and pyroglutamidation.
Simultaneous analysis of multiple proteins can hardly be
realized.
It’s single threaded and low efficient.