The Leaf THE LEAF Sept-October 2019 | Page 6

Once researchers had figured out when cannabis first diverged from a common ancestor, the question of where still remained. Paleobotanists then turned to microfossils, such as fossilised pollen, to fill in the records. They found that pollen from the closely related cannabis and hop plants are almost indistinguishable. To overcome this problem, scientists realised that because cannabis typically grows in open grasslands, and hops grow in forests, the pollen could be classified by identifying other plants that commonly occur alongside it. Researchers used plants that are typically seen in open grasslands to identify the fossilised pollen as cannabis. How Scientists Dated and Located Fossilised Cannabis Pollen Fossilised pollen is usually used to date the layer in which it is found, which tells a lot about the environment at the time. However, in this case, the pollen was the unknown. Researchers aged it with radiocarbon dating. Radiocarbon dating measures the amount of radiocarbon (C14) left in a fossilised animal or plant. C14 degrades at a known rate, and so by testing the amount of C14 left in a fossil, its age can be accurately calculated. By using this analysis, the oldest fossilised cannabis pollen was located in the Ningxia Province, China. Researchers dated the pollen at 19.6 million years old. But with cannabis diverging 27.8 million years ago, this date wasn’t close enough. Cannabis stems from a single location on the Tibetan Plateau Further research of the region and tracking of a plant called Artemisia, which has a close alliance and parallel evolutionary pattern to cannabis, pinpointed the north-eastern Tibetan Plateau as the cannabis centre of origin. At the time, the Tibetan Plateau created an environment that supports the theory that cannabinoids developed to protect the plant from UV rays and herbivores. These are both issues in the high altitude, open grassland Tibetan Plateau. Fossil pollen records tell us that cannabis dispersed into Europe 6 million years ago. Then later East into China 1.2 million years ago. By mapping the distribution of pollen over time, scientists were able to see that European cannabis went through repeated genetic bottlenecks. Following the warm and wet Holocene period, forests replaced open grasslands. Cannabis retreated to the small pockets of open space that it could inhabit. In these small and isolated areas, the population of cannabis shrank. These separated cannabis populations then evolved differently, eventually creating the separate and distinct landrace strains of the European-evolved sativa and the Asian- evolved Indica. By tracing cannabis evolution back to a single location on the Tibetan Plateau millions of years ago, we have uncovered the site of the original cannabis landrace strain. Over thousands of years, the original cannabis strain moved across continents, becoming isolated in certain areas. The original landrace strain had to then develop to new conditions, eventually leading to a variety of landrace strains. Each developed unique Geno-phenotypical characteristics reflective of adaptations provoked by their local environment. And these ancient strains have become the mythologised landrace strains that we idolize today. https://www.rxleaf.com/fossilized-cannabis-original- land-race-evolution/