The Leaf THE LEAF November-December 2019

Moreover, … treatment for 48 h, increased proliferation … Surprisingly, stimulation of CB1R … also promoted neuronal differentiation (without affecting glial differentiation), at 7 days, as shown by counting the number of NeuN- positive neurons in the cultures. cannabinoids) on hippocampal neurogenesis remain unknown. This study aimed at investigating the potential regulatory capacity of the potent synthetic cannabinoid HU210 on hippocampal neurogenesis and its possible correlation with behavioural change. Moreover, by … a method that allows the functional evaluation of neuronal differentiation, we observed an increase in neuronal-like cells. This proneurogenic effect was blocked when SVZ cells were co-incubated with … the CB1R antagonist AM 251, for 7 days, thus indicating that this effect involves CB1R activation. We show that both embryonic and adult rat hippocampal NS/PCs are immunoreactive for CB1 cannabinoid receptors, indicating that cannabinoids could act on CB1 receptors to regulate neurogenesis. This hypothesis is supported by further findings that HU210 promotes proliferation, but not differentiation, of cultured embryonic hippocampal NS/PCs likely via a sequential activation of CB1 receptors … In accordance with an effect on neuronal differentiation and maturation … also increased neurite growth … Taken together, these results demonstrate that CB1R activation induces proliferation, self- renewal and neuronal differentiation from mouse neonatal SVZ cell cultures. The Journal of Clinical Investigation November 2005 Cannabinoids promote embryonic and adult hippocampus neurogenesis and produce anxiolytic- and antidepressant-like effects Abstract The hippocampal dentate gyrus in the adult mammalian brain contains neural stem/progenitor cells (NS/PCs) capable of generating new neurons, i.e., neurogenesis. Most drugs of abuse examined to date decrease adult hippocampal neurogenesis, but the effects of cannabis (marijuana or Chronic, but not acute, HU210 treatment promoted neurogenesis in the hippocampal dentate gyrus of adult rats and exerted anxiolytic- and antidepressant-like effects. … suggesting that chronic HU210 treatment produces anxiolytic- and antidepressant-like effects likely via promotion of hippocampal neurogenesis.

The Leaf THE LEAF November-December 2019 | Page 14