The Leaf THE LEAF November-December 2017 | Page 6

Endocannabinoid Receptors – More Than Just CB1 and CB2 (Part 2) Receptor Dimerisation - Expanding the reach of Cannabinoids Cannabinoid receptors can link with other receptors and modify their function, opening a new avenue for understanding how cannabinoid exert their effects. I previously showed how endocannabinoids like anandamide can directly interact with receptors outside the cannabinoid system. However, this alone does not explain the multitude of effects that cannabinoids have. There is another important way that cannabinoids can interact with other systems – this is through receptor dimerisation. This process is turning out to be so important for how cannabinoids work – including both for beneficial health effects as well as for undesired side effects. For example, a potential role of cannabinoid receptor dimers has been explored in: • Tolerance to pain-blocking effects of opiates • Depression and anxiety in chronic pain • Negative effects of cannabis on memory • Parkinson’s and Huntington’s Disease • Cancer cell metastasis GPCR Dimerisation CB1 and CB2 belong to a class of receptors called G-protein coupled receptors (or GPCRs). These receptors were traditionally thought to function as independent units. Then it was discovered that two of the same GPCRs could come together in the cell membrane to form a receptor homodimer. The CB1 receptor homodimer was first characterised in a 2002 study, although the consequences of this remain unknown The next discovery was even more surprising – different types of GPCRs could bind each other to form a receptor heterodimer (also called a heteromer for short). This opened up an exponential number of ways that a single receptor (for example, the cannabinoid CB1 receptor) could influence other neurotransmitter systems. What can heterodimerization change about how the receptor functions? A lot of things: • Receptor signalling: Increasing or decreasing the signal generated from the receptor or even changing the signalling pathway activated by the receptor. • Ligand binding: Changing the affinity of a ligand for its receptor • Receptor Trafficking: Location of the receptor on the membrane or internalisation of the receptor into the cell I will highlight the effects of some key cannabinoid receptor dimers below: