The Journal of the Arkansas Medical Society Med Journal Sept 2019 FInal 2 | Page 17
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Figure 2: Dissection down to the PIF plane shows that the injectate spread throughout
the PIF plane entirely, proving involvement of the anterior cutaneous branches of the
intercostal nerve. Lateral spread of injectate is likely to involve the lateral cutaneous
branch of the intercostal nerve as well. In this figure the pectoralis major muscle (PMM)
is reflected bilaterally. A 12 cm ruler is placed in the midline over the sternum at the
base of the sternal notch. The sternal head of the sternocleidomastoid muscles (SH)
are indicated bilaterally.
METHODS
With the approval of the UAMS Institutional
Review Board, five lightly embalmed cadavers
were obtained from the Department of Anatomy.
The cadavers were embalmed in house using
the methods described by Anderson, stored at 4
degrees Celsius until the day of use, taken out the
morning of the procedure and allowed to warm to
room temperature for six-to-eight-8 hours. 8 Under
ultrasound guidance, methylene blue dye was
injected into the PIF plane of each hemi-chest wall
of each cadaver for a total of 10 hemi-chest walls.
For each left-side hemi-chest wall, a single
injection was performed at the level of the fourth
rib, into which 20mL of dye was injected. For each
right-side hemi-chest wall, two separate injections
at the third and fifth ribs were performed; 10mL of
dye was injected at each site. In total, each hemi-
chest wall was injected with 20mL of dye.
A draped, linear-array NextGen LOGIQ e
ultrasound transducer (GE Healthcare UK, Ltd.,
Chalfont, Buckinghamshire, UK) was used. The
ultrasound probe was placed 2 cm laterally from
the sternal border in the parasagittal plane, and the
indicated rib was identified. A two-inch echogenic
20-gauge block needle (B. Braun Medical Inc.,
Bethlehem, PA, USA) was then advanced using
an in-plane technique from cephalad to caudad
starting at the superior border of the probe until
the needle tip was seen between the pectoralis
major muscle (PMM) and the external intercostal
membrane (EIM). After a test dose of 1-2 mL of
normal saline to confirm correct needle placement
by hydrodissection, the indicated volume of blue
dye (equal parts 0.25% methylene blue dye with
normal saline) was injected through the needle.
(Fig. 1) After the injection was complete, the
injectate was allowed to settle for 20 minutes. An
experienced anatomist then dissected the chest
wall down to the PIF plane.
RESULTS
Dissection of the cadavers confirmed
successful injection in nine of the 10 hemi-chest
walls and showed that the dye spread entirely
throughout the PIF plane (Fig. 2). One injection of a
left hemi-chest wall failed due to incorrect needle-
tip placement within the PMM. The mean single-
injection (left side) maximal superior-inferior spread
was 13.75 cm (standard deviation 2.10 cm). The
mean double-injection (right side) maximal superior
inferior spread was 16.63 cm (standard deviation
3.67 cm). The mean single-injection maximal
lateral spread was 12 cm (standard deviation 2.16
cm). The mean double-injection maximal lateral
spread was 12.25 cm (standard deviation 1.70 cm).
The single-injection achieved a mean intercostal-
space spread of 4.75 spaces (standard deviation
0.5 spaces). The double-injection achieved a mean
intercostal-space spread of 5.75 spaces (standard
deviation 1.22 spaces).
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> Continued on page 66.
NUMBER 3
SEPTEMBER 2019 • 65