S . Robertson and K . White : J Extra Corpor Technol 2024 , 56 , 65 – 70 67
Figure 2 . In Vitro setup for Experiments 2 and 5 simulating volume transfused from a pressurized bag of saline , through a pressure transducer , to a bag full of 200 mL of saline and under 150 mmHg of pressure .
bag ( Ethox Medical , LLC , Grand Rapids , MI , USA ). The pressure bag was continually monitored throughout the experiment and kept at 300 mmHg utilizing the green 300 mmHg indicator . The saline bag was spiked using an Edwards TruWave 3 cc / VAMP 72 00 pressure monitoring set ( Edwards Lifesciences Corporation , Irvine , CA , USA ). The tubing below the transducer was discarded and a pressure line was added . The opposite end was connected to a BD MaxGuard bag access spike ( Becton , Dickinson and Company , Lakes , NJ , USA ). An empty 500 mL 0.9 % saline bag was spiked and all lines were primed using the Snap-Tab flushing device . This empty bag was hung at the same level as the pressurized bag . After 24 h , the passively shunted volume from the pressurized saline bag to the empty bag was removed and measured using a 50 mL syringe .
Experiment 2 ( 150 mmHg )
Experiment 2 was set up the same as experiment 1 with the following modifications : the empty 500 mL saline bag was filled with 200 mL of 0.9 % saline , placed into an Ethox 500 mL pressure infusion bag , and pressurized to 150 mmHg ( Figure 2 ). The pressure bag was continually monitored throughout the experiment and kept at 150 mmHg utilizing the white 150 mmHg indicator . After 24 h the 150 mmHg bag was depressurized and the initial 200 mL 0.9 % saline was removed . The remaining 0.9 % saline was removed and measured . Experiment 3 ( �50 mmHg )
Experiment 3 was set up the same as experiment 1 with the following modifications : the empty saline bag was removed and replaced with a Baxter Clearlink Systems buretrol solution set ( Baxter Healthcare Corporation , Deerfield , IL , USA ), a threeway stopcock was attached to the luer connector on the top of the buretrol solution set , and a pressure line was connected to a vacuum regulator with the opposite end attached to the stopcock ( Figure 3 ). Another pressure line was added to the remaining port of the stopcock with the opposite end connected