M . Sancheti et al .: J Extra Corpor Technol 2024 , 56 , 37 – 44 43
In this study , we were able to establish a laboratory protocol using bovine blood that minimizes the inter-variations within each type of circuit through different time points . The results presented in this report confirm that the method is precise , reliable , and reproducible given no inter-variation within each coating at different time points . The preliminary data collected in this study will be used for power analysis and sample size calculations to determine the proper sample size ( numbers for technical and biological replicates ) for future comparative studies .
Funding
This study was supported by Midwestern University Graduate Fund ( K . N ), college of Health Sciences Faculty Intermural Grant ( N . D .), and Arizona College of Osteopathic Medicine KAS Summer Research Fellowship Award ( M . S .).
Conflict of interest The authors declare no conflict of interest .
Data availability statement
The raw original data used in this study is available by the authors upon request .
Authors contribution statement
MS contributed to data generation , data analysis , and manuscript writing . MR had equal contribution to data generation , analysis , and manuscript writing . CB helped with experimental design , data analysis , and data presentation . WL conducted the ELISA test and helped with data generation and analysis . KN helped with experimental protocol and data collection . TR helped with data interpretation and manuscript writing . ME and ND are senior investigators involved in experimental design , data analysis and review , manuscript writing and editing .
Ethics approval
The study does not involve animal or human subjects . The biosafety protocol was approved by Midwestern University Office of Research & Sponsored Program .
Supplementary material
The Supplementary materials of this article are available at https :// ject . edpsciences . org / 10.1051 / ject / 2024003 / olm .
Supplementary Figure S1 . Raw values for platelet activation biomarkers using Trillium Ò Biosurface bypass . Bar graphs presenting raw values of platelet activation markers in collected bovine blood at 5- , 30- , and 55-minutes using Trillium Ò Biosurface bypass circuit without normalization over the platelet counts . ( Mean ± SD , P < 0.05 , five technical replicates ). GPIIb / IIIa : Glycoprotein IIb / IIIa ; PF4 : Platelet Factor 4 ; b-TG : b-Thromboglobulin
Supplementary Figure S2 . Raw values for platelet activation biomarkers using Xcoating TM bypass . Bargraphs presenting raw values of platelet activation markers in collected bovine blood at 5- , 30- , and 55-minutes using Xcoating TM bypass circuit without normalization over the platelet counts . ( Mean ± SD , P < 0.05 , five technical replicates ). GPIIb / IIIa : Glycoprotein IIb / IIIa ; PF4 : Platelet Factor 4 ; b- TG : b-Thromboglobulin
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