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CHITOSAN MODIFIED POLY( LACTIC-CO-GLYCOLIC) ACID NANOPARTICLES INTERACTION WITH NORMAL, PRECANCEROUS KERATINOCYTES AND DENTAL PULP CELLS

Original Articles

5. Conclusions This study offers new insight on NPs uptake within human oral cells. PLGAChi NPs are not suitable in applications regarding DPCs, as they do not enter these cells. But, PLGAChi NPs are internalised by both human keratinocytes and fibroblasts. Chitosan-coated PLGA NPs have proved to be potent in crossing the cellular membrane of epithelial cells. Therefore, PLGAChi NPs are highly recommended for being used in drug delivery systems to the oral mucosa. This promising results suggest the need for further studies regarding PLGAChi NPs, and its uses in oral mucosa diseases or anticancer therapy. In conclusion, more research is needed to fully explore the underlying mechanisms of celllular uptake of PLGA with chitosan surface modification.
Author Contributions MJRV contributed to the concept of the article, data gathering, data analysis and interpretation. BC’ s contribution was very important in the concept, interpretation and critical revision of the manuscript. DEC contributed to establishing of protocols, data gathering, interpretation of the
References
1. Li B, Li Q, Mo J, Dai H. Drug-loaded polymeric nanoparticles for cancer stem cell targeting. Front Pharmacol. 2017; 8:51. doi: 10.3389 / fphar. 2017. 00051. eCollection 2017.
2. Fonseca AC, Ferreira P, Cordeiro RA, et al. Drug delivery systems for predictive medicine: polymers as tools for advanced applications. Mozaffari MS, editor. In: New Strategies to Advance Pre / Diabetes Care: Integrative Approach by PPPM, Advances in Predictive, Preventive and Personalised Medicine 3. Springer Science + Bussines Media Dordrecht; 2013.
3. Fonseca AC, Serra AC, Coelho JF. Bioab-sorbable polymers in cancer therapy: latest developments. EPMA J. 2015; 6:22. doi: 10.1186 / s13167-015-0045-z. eCollection 2015.
4. Virlan MJR, Miricescu D, Totan A, et al. Current uses of poly( lactic-co-glycolic acid) in the den-tal field: A comprehensive review. Journal of Chemistry, 2015, Article ID 525832, http:// dx. doi. org / 10.1155 / 2015 / 525832.
5. Ravi Kumar MN, Bakowsky U, Lehr CM. Preparation and characterization of cationic PLGA nanospheres as DNA carriers. Biomaterials. 2004; 25( 10): 1771-1777.
6. Dyawanapelly S, Koli U, Dharamdasani V, Jain R, Dandekar P. Improved mucoadhesion and cell uptake of chitosan and chitosan oligosaccharide surface-modified polymer nanoparticles for mucosal delivery of proteins. Drug Deliv. and Transl. Res. 2016; 6( 4): 365-379. doi: 10.1007 / s13346-016-0295-x.
7. Makita-Chingombe F, Kutscher HL, DiTursi SL, Morse GD, Maponga CC. Poly( lactic-co-glycolic) Acidchitosan dual loaded nanoparticles for antiretroviral nanoformulations. J Drug Deliv. 2016; 2016:3810175. doi: 10.1155 / 2016 / 3810175.
8. Kawashima Y, Yamamoto H, Takeuchi H, Kuno Y. Mucoadhesive DL-lactide / glycolide copolymer nanospheres coated with chitosan to improve oral delivery of elcatonin. Pharm Dev Technol. 2000; 5( 1): 77- 85. results and critical revision of the manuscript. MRC critically revised the manuscript. MG contributed to all stages of the article from the concept of the article, protocols, interpretation and critical revision of the manuscript. All authors approved the final version of the article.
Acknowledgments Bogdan Calenic acknowledges that this work was supported by a grant from the Romanian National Authority for Scientific Research and Innovation, CNCS – UEFISCDI, project number PN- II-RU-TE-2014-4-1879. We thank Professor Sabliov CM, Agricultural and Biological Engineering Department, Louisiana State University and LSU Ag Center, Baton Rouge, LA, USA for providing us with the PLGAChi nanoparticles used in this study. We acknowledge Dr. G Negroiu, Institute of Biochemistry, Bucharest, Romania for assisting us in the acquisition of microscopy images. Many thanks to Macedon SE from Institute of Electrical and Electronics Engineers for the help in the quantification of the uptake of nanoparticles inside cells.
9. Chronopoulou L, Nocca G, Castagnola M, et al. Chitosan based nanoparticles functionalized with peptidomimetic derivatives for oral drug delivery. N Biotechnol. 2016; 33( 1): 23-31. doi: 10.1016 / j. nbt. 2015.07.005.
10. 10. Bellamy C, Shrestha S, Torneck C, Kishen A. Effects of a bioactive scaffold containing a sustained transforming growth factor-β1-releasing nanoparticle system on the migration and differentiation of stem cells from the apical papilla. J Endod. 2016; 42( 9): 1385-1392. doi: 10.1016 / j. joen. 2016.06.017.
11. Shrestha S, Torneck CD, Kishen A. Dentin conditioning with bioactive molecule releasing nanoparticle system enhances adherence, viability, and differentiation of stem cells from apical papilla. J Endod. 2016; 42( 5): 717-723 doi: 10.1016 / j. joen. 2016.01.026.
12. Lee BS, Lee CC, Wang YP, et al. Controlled-release of tetracycline and lovastatin by poly( D, L-lactideco-glycolide acid)-chitosan nanoparticles enhances periodontal regeneration in dogs. Int J Nanomedicine. 2016; 11:285-297. doi: 10.2147 / IJN. S94270. eCollection 2016.
13. Shrestha A, Kishen A. Antibacterial nano-particles in endodontics: A review. J Endod. 2016; 42( 10): 1417-1426. doi: 10.1016 / j. joen. 2016. 05.021.
14. Del Carpio-Perochena A, Kishen A, Shrestha A, Bramante CM. Antibacterial properties associated with chitosan nanoparticle treatment on root dentin and 2 types of endodontic sealers. J Endod. 2015; 41( 8): 1353-1358. doi: 10.1016 / j. joen. 2015.03.020.
15. Del Carpio-Perochena A, Bramante CM, Duarte MA, de Moura MR, Aouada FA, Kishen A. Chelating and antibacterial properties of chitosan nanoparticles on dentin. Restor Dent Endod. 2015; 40( 3): 195-201. doi: 10.5395 / rde. 2015.40.3.195.
16. Mazzarino L, Borsali R, Lemos-Senna E. Mucoadhesive films containing chitosan-coated nanoparticles: a new strategy for buccal curcumin release. J Pharm Sci. 2014; 103( 11): 3764-3771. doi: 10.1002 / jps. 24142.

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