CHITOSAN MODIFIED POLY( LACTIC-CO-GLYCOLIC) ACID NANOPARTICLES INTERACTION WITH NORMAL, PRECANCEROUS KERATINOCYTES AND DENTAL PULP CELLS
Original Articles
5. Conclusions This study offers new insight on NPs uptake within human oral cells. PLGAChi NPs are not suitable in applications regarding DPCs, as they do not enter these cells. But, PLGAChi NPs are internalised by both human keratinocytes and fibroblasts. Chitosan-coated PLGA NPs have proved to be potent in crossing the cellular membrane of epithelial cells. Therefore, PLGAChi NPs are highly recommended for being used in drug delivery systems to the oral mucosa. This promising results suggest the need for further studies regarding PLGAChi NPs, and its uses in oral mucosa diseases or anticancer therapy. In conclusion, more research is needed to fully explore the underlying mechanisms of celllular uptake of PLGA with chitosan surface modification.
Author Contributions MJRV contributed to the concept of the article, data gathering, data analysis and interpretation. BC’ s contribution was very important in the concept, interpretation and critical revision of the manuscript. DEC contributed to establishing of protocols, data gathering, interpretation of the
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Acknowledgments Bogdan Calenic acknowledges that this work was supported by a grant from the Romanian National Authority for Scientific Research and Innovation, CNCS – UEFISCDI, project number PN- II-RU-TE-2014-4-1879. We thank Professor Sabliov CM, Agricultural and Biological Engineering Department, Louisiana State University and LSU Ag Center, Baton Rouge, LA, USA for providing us with the PLGAChi nanoparticles used in this study. We acknowledge Dr. G Negroiu, Institute of Biochemistry, Bucharest, Romania for assisting us in the acquisition of microscopy images. Many thanks to Macedon SE from Institute of Electrical and Electronics Engineers for the help in the quantification of the uptake of nanoparticles inside cells.
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