CHITOSAN MODIFIED POLY(LACTIC-CO-GLYCOLIC) ACID NANOPARTICLES INTERACTION WITH
NORMAL, PRECANCEROUS KERATINOCYTES AND DENTAL PULP CELLS
20
E. POE9i 200 µg/mL PLGAChiNPs 24h.
F. Detection of PLGAChi NPs internalization inside
POE9i (image of POE9i 200 µg/mL PLGAChi NPs 12h).
Figure 2. Fluorescence images showing uptake of PLGAChi NPs inside POE9i. The fluorescent green dots are the
internalized PLGAChi NPs.
A. DPCs Control.
B. DPCs 200 µg/mL PLGAChi NPs 24h.
Figure 3. Fluorescence microscopy images showing no signs of PLGAChi NPs uptake by DPCs cells.
Figure 4. Confocal microscopy image demonstrating
PLGAChi NPs uptake inside NOKs. The green
fluorescent PLGAChi NPs are observed inside cells. Figure 5. Confoccal Images of POE9i exposed to
200 µg / mL PLGAChi NPs for 12H. The fluorescent
PLGAChi NPs green NPs are observed inside the cells.
3.3. Cytotoxicity evaluation of PLGAChi NPs in
NOKs and POE9i cells
After 24h exposure to PLGAChi NPs, NOKs
demonstrated no significant difference in the
viability values at all tested concentrations: 5μg/
mL, 20 μg/mL and 200 μg/mL PLGAChi NPs. A slight decrease in viability was observed inthe
POE9i cell line exposed to 20 μg/mL PLGAChi
NPs. However, the POE9i samples exposed to 20
μg/mL PLGAChi NPs showed 81% percentage of
viable cells, as compared to 88 % live cells, in the
control sample.
Stoma Edu J. 2017;4(1): 16-26. http://www.stomaeduj.com