Speciality Chemicals Magazine MAR / APR 2023 | Page 30

Consuelo García and Eva Gómez of Lubrizol Life Science Beauty share study results on an exposome protection natural active ingredient *

New biotechnological ingredient for multi-ethnic use with exposome protection

Consuelo García and Eva Gómez of Lubrizol Life Science Beauty share study results on an exposome protection natural active ingredient *

Consumers are growing more concerned about not only the impact of pollution on human health , but also the impact of other environmental exposures . In 2005 , epidemiologist Christopher P . Wild coined the term ‘ exposome ’ to refer to the lifelong totality of exposure to environmental and lifestyle factors .

Later , the concept would be adapted to dermatology , which marked a shift in the way scientists viewed the impact of environmental factors on the skin . 1 Until that point , the impact of these factors had been studied separately and molecular crosstalk was poorly understood . 2 , 3
However , when the impact of several environmental aggressors was combined – for example , air pollutants and UV radiation , or temperature and UV radiation4 – the results could not be explained by the cumulative effect of each of the factors separately . This suggested that there may be synergistic effects and molecular crosstalk , which could only be elucidated through an exposome approach .
This article gives details of the first study in the cosmetic field to look at the combined effects of UV radiation , air pollution and greenhouse damage , as well as pollen , on the skin , scalp and hair . We also share information about Xpozuki Biotech Ingredient **, Lubrizol Life Science Beauty ’ s new active , which was used in the study .
This has been shown to protect , defend and repair damage caused by these exposome factors .
Greenhouse stress damage testing
Initially , our team took human dermal fibroblasts ( HDFa ) and human epidermal keratinocytes ( HEKa ) co-culture cells and seeded them in 96-well plates in the culture medium . After 24 hours of incubation at 37 º C in 5 % CO 2
-humidified air , the cell culture medium was removed and fresh medium was added , with Xpozuki Biotech Ingredient solubilised at 1 % or 2 % ( v / v ) for one hour .
Cells were then exposed to particulate matter PM2.5 ( Diesel particulate matter NIST1650b ), followed by solar irradiation for two hours , in the presence of the active . Next , they were incubated at 38 º C in 20 % CO 2
-humidified air for two hours ( greenhouse stress damage ) in the presence of the active . Control cells were maintained at room temperature and protected from light during the irradiation time and were incubated at normal conditions , 37 º C in 5 %
CO 2
-humidified air .
Figure 1 - Normalised viability on HEKa-HDFa co-culture after incubation with 1 % & 2 % natural active ingredient at 1 % and 2 % and exposed to exposome stress damage .
Note : Data shown as mean ± SEM of the mean of 4 & 3 independent experiments respectively . Statistical significance ; **** p < 0.0001 ; ** p < 0.01 calculated using one-way ANOVA test
30 SPECIALITY CHEMICALS MAGAZINE ESTABLISHED 1981