DNA Isolation from different Trace Materials
Isolation of DNA out of different trace materials
becomes more and more important in the field of
forensics.
For more information on
Hamilton Robotics products,
contact
[email protected]
Starting with lysed trace material, the fully
automated process on the MICROLAB STAR includes
DNA extraction, quantification, normalization and
PCR set-up. For DNA extraction out of trace materials
like cigarettes, sputum and hairs, the ChargeSwitch®
Forensic DNA Purification Kit from Invitrogen is used.
After isolation, the DNA is quantified using
fluorescence dyes with an integrated BMG FLUOstar
plate reader. Finally, the DNA is normalized and used
for
PCR
set-up.
The DNA concentration is measured in a range of
7pg/µl to 1000pg/µl. Samples with a concentration
lower than 30pg/µl are excluded from PCR set-up in
order to save expensive chemistry. The complete
processing of 96 samples is finished within 4h.
The data handling procedure is customized and
ensures that the samples are traced throughout the
whole process. A text file with the sample
information is used as an input file, and an output file
for documentation is generated at the end of the
process.
Hamilton Robotics workstations make use
of FluidX sample storage consumables and
Macherey-Nagel nucleic acid extraction
kits.
has placed 40 Hamilton systems in South Africa
since January 2011, and has 8 dedicated Hamilton engineers for all
required solutions.