Seagrass-Watch Magazine Issue 47 - March 2013 | Page 20

Sprigs Sprigs (bare-root transplant units consisting of small rhizome sections with a small number of leaf-bearing shoots and rhizome/root nodes) of Cymodocea rotundata, C. serrulata, Halodule uninervis, Syringodium isoetifolium and Thalassia hemprichii were selectively removed from a donor meadow by hand. Bundles of sprigs were then planted into a hole in the sediment created using a crowbar. Beside each sprig was laid a bamboo stake as a reference point. Unfortunately, only shoots of Thalassia survived in the transplant site. Bricks were mounted at each corner of the frame for ballast. At the transplant site, the bamboo frames were inverted and dropped to the seafloor (i.e. roots and rhizomes into the substrate and the leaves pointing to the water surface). The frame was removed once the plastic ties had degraded after a month and the shoots had anchored into the sediment. Using the TERF technique, only T. hemprichii survived – all other species died. Limited success: using the TERF technique, only T. hemprichii survived – all other species died. Success: Transplanted sprigs of Thalassia hemprichii surrounded by single shoots of Enhalus acoroides. TERFS method In this method, a weighted bamboo frame, to which seagrass shoots are tied with biodegradable plastic, was dropped onto the transplant site. TERFS are usually made from a steel frame, but this was expensive and prone to theft. Bamboo stakes on the other hand were inexpensive, easy to work and safe from theft. The frames were made of bamboo stakes 2cm diameter and 40 60 cm long. They are arranged in a 10cm grid and tied with plastic at the meeting point. Shoots of T. hemprichii, C. rotundata, C. serrulata, H. uninervis and S. isoetifolium were collected from a donor meadow, and their leaves were trimmed to 2cm of the leaf midrib. Seagrass shoots (25 per frame) were mounted up