Saliksikhay SALIKSIKHAY_abstracts_PDFv5 | Page 48

Transport Solutions & Energy Local bioethanol plants have an installed production capacity of 365 million liters. However, the actual amount of bioethanol they produce only amounts to 270 million liters—well below the 550 million liters required for a 10% blend needed for gasoline. The shortfall is due to the limited supply of feedstock in the country, which consists of mainly sugarcane and molasses. Nipa and sweet sorghum are being tapped to expand the feedstock mix, but another solution is second generation bioethanol from lignocellulosic material, which is seen to provide greater diversity while avoiding the food vs. fuel debate. This project explored the consolidated bioprocessing (CBP) of lignocellulosic material for bioethanol production. CBP combines three separate process (enzyme production, substrate hydrolysis, and fermentation) into a single step process. A fungus, Fusarium moniliforme, was found to produce enzymes that could convert glucose and xylose to ethanol, in addition to its ability to hydrolyze or depolymerize cellulose. The process and fungus were tested on various biomass, namely pretreated napier grass, rice straw, banana pseudostem, and coconut coirunder. Various pretreatments such as organosolve using formic acid, combined formic and acetic acids, alkali, hydrogen peroxide, and COSLIF, were used and compared on banana pseudostem. Formic acid pretreatment was found to be most effective in dissolving lignin and opening up the pores of the biomass to allow access for the enzymes into the cellulose fibrils. Recovery of the formic acid and dissolved lignin from the effluent was also optimized. The processes developed by the researchers could provide for an alternative and more efficient method of producing ethanol locally, reducing the country’s dependence on bioethanol importation for vehicular fuel needs. The research was conducted with the help of undergraduate BS ChE students (graduated): Ariel Raye Rica, John Steven Magboo, and Almajoy Ilao (BS ChE 2016) Kimberly R. Palomique, Dave E. Ramos, and Troy Giuseppe . Tolentino (BS ChE 2017) Kimberly Louise Y. Co, Luke R. Magsanoc, and Abigail Laureen C. Sy (BS ChE 2018) Aaron John Medina, Jun Bryan Juen, and Shayne Rae Primavera (BS ChE 2019) Emilio Paul Nogales, Grace Iris Miranda, and Ronald Eldrick Guico Partially funded by: DOST ERDT (thesis grant of Mr. Lao) Various UP Engineering Research and Development Foundation Inc. thesis grant donors 44 | UP Diliman SALIKSIKHAY