joints, and (3) to determine serum and synovial fluid SAA levels of normal
equine joints and osteoarthritic joints.
Platelet-rich product was prepared from 10 horses using a gravity filtration
system, namely Equine Platelet Enhancement TherapyTM (E-PET, Pall
Corporation, Port Washington, NY). Horses consisted of two groups, OAaffected group (n=5) and control group (n=5). Joints included were
metacarpophalangeal and carpal joints. Clinical assessments, cytologic
analysis of synovial fluid and complete blood counts were performed at day 1,
2, 5, 21 and 56 after injection. Platelet-rich product composition and growth
factor content (PDGF and TGF-β) were analyzed.
The gravity filtration system produced a moderately concentrated Platelet-rich
product. At day 1 after injection, all Platelet-rich treatments caused a
significant increase in synovial WBC concentration and neutrophil percentage.
Platelet-rich product can safely be used to treat equine joints. A Platelet-rich
product can be prepared using a gravity filtration system, eliminating the need
for centrifugation.
References
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Sutton, S., Clutterbuck, A., Harris, P., Gent, T., Freeman, S., Foster, N., Barrett-Jolley, R. and Mobasheri,
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the pathogenesis of osteoarthritis. The Veterinary Journal 179, 10-24.
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Jacobsen, S., Thomsen, M.H. and Nanni, S. (2006) Concentrations of serum amyloid A in serum and
synovial fluid from healthy horses and horses with joint disease. American Journal of Veterinary Research
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Jacobsen, S., Thomsen, M.H. and Nanni, S. (2006) Concentrations of serum amyloid A in serum and
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