SAEVA Proceedings 2014 | Page 51

46TH  ANNUAL  CONGRESS  OF  THE  SAEVA        SKUKUZA      16-­‐20  FEBRUARY  2014   51     f) CEM was absent in Arabian and American Saddlehorses in SA 2. Bacteriology and pathogenesis a) CEM was not exclusively a venereal disease b) CEM was apparently readily contagious c) CEM may have persisted for an undefined interval outside the genital tract environment d) CEM was transmissible via AI using semen diluted with antibiotic-containing extender 3. Disease prevention a) CEM control was supported by the current post-entry quarantine protocol 4. Diagnostic methods a) qPCR enabled accurate practical, high-throughput, rapid and economical identification of T. equigenitalis b) In-treatment qPCR improved therapeutic protocols for T. equigenitalis elimination c) Stallion screening was a practical and rapid method to ascertain the prevalence of T. equigenitalis d) Repeat sampling increased the sensitivity of T. equigenitalis detection e) T. equigenitalis had the greatest predilection for the urethral fossa and sinus in stallions 5. Treatment and management of carrier animals a) The efficacy and duration of previously-advocated treatment protocols was undefined b) A treatment protocol to eliminate T. equigenitalis was objectively evaluated c) The treatment process may itself enhance dispersal of T. equigenitalis d) Topical treatment for T. equigenitalis predisposed to genital colonisation by other bacterial pathogens Conclusions CEM outbreak reports are increasingly associated with artificial breeding in nonThoroughbreds possibly in association with shortcomings in biosecurity protocols associated with semen collection, processing and shipping. The role of contaminated   51