SAEVA Proceedings 2014 | Page 126

126   46TH  ANNUAL  CONGRESS  OF  THE  SAEVA        SKUKUZA      16-­‐20  FEBRUARY  2014     alerted observers to possible cases of clinical infection. A set of duplicate nasal swabs was again collected from each horse prior to departure from the sales complex. Any horses that showed elevated rectal temperature (> 38.5 ºC) measured with a Thermoval® rapid digital thermometer and, or a nasal discharge underwent a clinical examination and serial nasal swab collection for qPCR analysis until departure. Results In 2011, two horses (from the E. Cape) showed molecular evidence of EHV-4 shedding and concurrent pyrexia during the sales period. The mean body temperatures showed significant elevations overall and for each farm. In 2013, no incidents of EHV-1 shedding and 21 incidents of EHV-4 shedding were recorded from 13 (13.9%) horses; that ranged from one to four incidents per horse. These animals originated from seven farms located as follows: W. Cape (n=3 farms; 6 horses), E. Cape (n=2 farms; 5 horses) and KZ-N (n=2 farms; 2 horses). At least one incident of nasal discharge in 86 (95.6%) and pyrexia in 49 (54.4%) was observed in the study population during the period of observation. In only a single horse was there not a single incident of pyrexia or nasal discharge recorded. The distribution of EHV-4 incidents by date showed that the first incidents were recorded on the second day after arrival with a gradual increase in frequency being recorded towards the end of the sales’ period. Discussion In the initial study in 2011, sales consignment of young Thoroughbreds was associated with elevations in both physiological stress and body temperature, with only limited shedding of EHV-4 observed. These findings did however support both the variables and the sampling methods and justified a follow-up study to define the prevalence o b6