ReMed 2018 ReMed Magazine N°4 - Cutting Edge | Page 15

When Cancer Catches the Flu Rihab FELLAH Historically, cancer was thought to be caused by viruses, though this remains true for some types. Today we are using those same agents to fight cancer. Indeed, virotherapy is at the forefront of the latest advancements in Microbio- logy, Immunology and Molecular Biology and offers interesting prospects in the field of oncology. V irus have always been known to be remar- kable microbes. From the Influenza Virus to the HIV, they have ability to replicate inside cells by exploiting cellular resources. They can cause at the end of their reproductive cycle a lysis of the host cell, which makes them one of nature’s most effective killers. Science, however, has found a way to exploit this skill in a more efficient way: killing cancer cells. Making, therefore, oncolytic viruses (OVs) the latest leap in oncology and its armamentarium. This encounter goes back to the early to mid-20th century. When regressions of tumoral growth have been observed in cancer patients suffering from viral infections. Though these regressions were temporary and rarely complete, they couldn’t but spark some hope that a novel cancer therapy has been discovered. The pursuit of such thought was halted due to the little understanding researchers had at the time of carcinogenesis and microbiolo- gy. Also, the great success that had been demons- trated by chemotherapy and radiotherapy in trea- ting cancer prevented it too. Today, in the era of genetic engineering and recombinant DNA tech- nology, viruses can be manipulated to be less har- mful towards healthy cells and to infect tumoral ones in a specific way. They offer a more targeted way of treating cancer than that found in chemo and radiotherapy, with minimal side effects. In 2015, researchers were at the cutting edge of cancer therapy when T-VEC–a genetically modi- fied HSV type 1– became the first oncolytic virus to be approved by the FDA (Food and Drug Admi- nistration) and the EMA (European Medical Agen- cy) for the treatment of unresectable melanoma. How do oncolytic viruses work exactly? They have many mechanisms of action. For starters, OVs whether natural or genetically engineered and/or modified are, by definition, viruses that have a particular tropism to cancer cells. They are incapable of replicating elsewhere. In fact, many OVs are modified to express mem- brane proteins/receptors that interact with coun- terparts found only in tumoral cells. Not forget- ting that these cells express little self-antigens such as HLA-1 and do not secrete IFN type I , II or TNF ( key cytokines for antiviral immunity) when infected. The lack of effective cornerstones such as these makes the antiviral immune response deficient in cancer cells, which explains their sus- ceptibility to viral infections. It has been known that cancer cells, escape the immune system via the expression of certain in- hibitory immune check points such as PD1 (Pro- grammed Death 1) and CTLA4 (Cytotoxic T Lym- phocyte Associated-protein 4). These molecules are what makes cancer cells to- lerated by the immune system. Many monoclonal antibodies targeting them have been developed and approved as cancer therapies for tumors such as melanoma and offer, thus, the possibility of a combined therapy with OVs. Viruses can also be engineered to produce growth factors that stimulate antitumoral immunity. It is the case with T-VEC which expresses GM CSF (Gra- nulocyte Macrophage Colony Stimulating Factor). OVs can also be made to activate intracellular pa- thways that induce apoptosis or deactivate those responsible for carcinogenesis. This is observed in Onyx 015, an engineered adenovirus that has the ability to activate the tumor suppressor p53 and therefore cause oncolysis. Though this particular virus has not been appro-