Product Literature Vol 3 Optimax Genotyping | Page 3

WT
P gene

GeneP 51.7ºC
1- Ladder
2- WT (wild type)
3- P transgene + WT
4- P transgene +WT
Controls were from previously genotyped animals
displaying either a P transgene or a Wild Type (WT)
band. (Not depicted here)

T gene
WT

GeneT 65ºC
1- Ladder
2- F1/F1 (homozygous of T gene)
3- Het (one copy of T gene + WT)
4- F1/F1 (homozygous of T gene)
Controls were from previously genotyped animals displaying
either a WT or both WT and T gene bands. (Not depicted
here)

Similar PCR product yields were obtained from both
the thermal cycler and the MultiGene Optimax
thermal cycler. When the traditional gradient cycler
was used for three separate PCR experiments, the
entire study was performed in approximately nine
hours. In contrast, equivalent results were obtained
with the MultiGene Optimax in approximately three
hours.

With the ability to perform various PCR reactions
with multiple annealing temperatures in a current
manner, the MultiGene OptiMax is the ideal
instrument for improved PCR efficiency in
genotyping labs.

We wish to thank Labnet International for
supplying the Enduro GDS imager, Enduro 300V
power supply and the OptiMax Thermal Cycler.
Furthermore, we would like to thank New England
Biolabs, Inc. and BioExpress for donating product
for this application note.

WT
D gene

GeneDR 60ºC
1- Ladder
2- DR/DR (homozygous of D gene)
3- Het (one copy of D gene + WT)
4- HET
Controls were from previously genotyped animals
displaying either homozygous for D gene or both WT and
D gene bands.(Not depicted here).

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